Willaertia magna De Jonckheere et al.

Willaertia magna De Jonckheere et al.

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编号:TS187677

品牌:Testobio

产品名称：	Willaertia magna De Jonckheere et al.
商品货号：	TS187677
Deposited As：	Protonaegleria westphali Michel
Strain Designations：	NI4C1(1)
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format：	frozen
Storage Conditions：	Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Axenic/Xenic：	Xenic
Type Strain：	no
Comments：	ultrastructure
Medium：	ATCC^® Medium 997: Fresh water ameba medium ATCC^® Medium 711: PYB
Growth Conditions：	Temperature: 25°C
Cryopreservation：	Cryoprotective Solution DMSO, 1.5 ml Pages Balanced Salt Solution (or similar), 8.5 ml Harvest and Preservation Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat. Harvest cells from a culture which is at or near peak density by adding 5 ml ATCC medium 1323 (Pages Balanced Salt Solution) and washing cells into suspension.xa0 Rub the surface of the plate with a spread bar to detach adhering trophozoites. Adjust the concentration of cells to at least 2 x 10⁶/ml in fresh medium. Mix the cell preparation and the cryoprotective solution in equal portions. Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation). Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0 Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator. To establish a culture from the frozen state place the vial in a 35°C water bath.xa0 Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.xa0xa0 Immediately after thawing, aseptically transfer the contents of the ampule to the center of a fresh plate of ATCC medium 997.xa0 Distribute the material evenly over the plate using a spread bar. Wrap the entire edge of the plate with parafilm and incubate upright at 25°C. Follow the protocol for maintenance of culture.
Name of Depositor：	R Michel
References：	Michel R, et al. Ultrastructure of the amoebo-flagellate Protonaegleria westphali. Parasitol. Res. 74: 23-29, 1987. PubMed: 3438290

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Willaertia magna De Jonckheere et al.

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产品名称：	Willaertia magna De Jonckheere et al.
商品货号：	TS187677
Deposited As：	Protonaegleria westphali Michel
Strain Designations：	NI4C1(1)
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format：	frozen
Storage Conditions：	Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Axenic/Xenic：	Xenic
Type Strain：	no
Comments：	ultrastructure
Medium：	ATCC^® Medium 997: Fresh water ameba medium ATCC^® Medium 711: PYB
Growth Conditions：	Temperature: 25°C
Cryopreservation：	Cryoprotective Solution DMSO, 1.5 ml Pages Balanced Salt Solution (or similar), 8.5 ml Harvest and Preservation Mix the components in the order listed. When the medium is added to the DMSO the solution will warm up due to chemical heat. Harvest cells from a culture which is at or near peak density by adding 5 ml ATCC medium 1323 (Pages Balanced Salt Solution) and washing cells into suspension.xa0 Rub the surface of the plate with a spread bar to detach adhering trophozoites. Adjust the concentration of cells to at least 2 x 10⁶/ml in fresh medium. Mix the cell preparation and the cryoprotective solution in equal portions. Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation). Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1 C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.) xa0 Ampules are stored in either the vapor or liquid phase of a nitrogen refrigerator. To establish a culture from the frozen state place the vial in a 35°C water bath.xa0 Immerse the vial to a level just above the surface of the frozen material. Do not agitate the vial.xa0xa0 Immediately after thawing, aseptically transfer the contents of the ampule to the center of a fresh plate of ATCC medium 997.xa0 Distribute the material evenly over the plate using a spread bar. Wrap the entire edge of the plate with parafilm and incubate upright at 25°C. Follow the protocol for maintenance of culture.
Name of Depositor：	R Michel
References：	Michel R, et al. Ultrastructure of the amoebo-flagellate Protonaegleria westphali. Parasitol. Res. 74: 23-29, 1987. PubMed: 3438290

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