| 产品名称： | PNF400 7990, pPM-7990 |
|---|---|
| 商品货号： | TS187812 |
| Designations： | PNF400 7990, pPM-7990 |
| GenBank Number： | U18917 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | MV Olson, L Riles |
| Vector： | Construct size (kb): 15.0 |
| Insert： | DNA: genomic Insert lengths(kb): 9.5 Gene product: non-specific DNA binding protein SPT2 |
| Insert Size (kb)： | 9.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Related Products： | component of:ATCC 77284 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 4.762, 0.408, 0.702, 0.902. Restriction digests of the clone give the following sizes (kb): EcoRI--9.0, 6.8; HindIII--9.8, 4.8, 0.7, 0.4; EcoRI/HindIII--9.0, 4.8, 0.9, 0.7, 0.4; PvuI--12.0, 3.2. The first 6.6 kb of the construct measured from the YIp5/yeast DNA junction contains the following restriction sites (kb from the HindIII site): PvuI--2.3; BamHI--4.0; HindIII--4.7, 5.1, 5.8; EcoRI--6.6. Overlaps clones with ATCC number(s): 70867. The RAD4 gene is contained within the 4.8 kb EcoRI/HindIII fragment. Isolated after plasmid rescue as an intramolecular ligation of a SalI fragment. Consists of YIp5 from SalI to the HindIII cloning site of the original integrant pR169, spt2 starting at the HindIII site, and the complete RAD4 gene. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Fleer R, et al. RAD4 gene of Saccharomyces cerevisiae: molecular cloning and partial characterization of a gene that is inactivated in Escherichia coli. Mol. Cell. Biol. 7: 1180-1192, 1987. PubMed: 3550431 Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151 |
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| 产品名称： | PNF400 7990, pPM-7990 |
|---|---|
| 商品货号： | TS187812 |
| Designations： | PNF400 7990, pPM-7990 |
| GenBank Number： | U18917 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | MV Olson, L Riles |
| Vector： | Construct size (kb): 15.0 |
| Insert： | DNA: genomic Insert lengths(kb): 9.5 Gene product: non-specific DNA binding protein SPT2 |
| Insert Size (kb)： | 9.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Related Products： | component of:ATCC 77284 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 4.762, 0.408, 0.702, 0.902. Restriction digests of the clone give the following sizes (kb): EcoRI--9.0, 6.8; HindIII--9.8, 4.8, 0.7, 0.4; EcoRI/HindIII--9.0, 4.8, 0.9, 0.7, 0.4; PvuI--12.0, 3.2. The first 6.6 kb of the construct measured from the YIp5/yeast DNA junction contains the following restriction sites (kb from the HindIII site): PvuI--2.3; BamHI--4.0; HindIII--4.7, 5.1, 5.8; EcoRI--6.6. Overlaps clones with ATCC number(s): 70867. The RAD4 gene is contained within the 4.8 kb EcoRI/HindIII fragment. Isolated after plasmid rescue as an intramolecular ligation of a SalI fragment. Consists of YIp5 from SalI to the HindIII cloning site of the original integrant pR169, spt2 starting at the HindIII site, and the complete RAD4 gene. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Fleer R, et al. RAD4 gene of Saccharomyces cerevisiae: molecular cloning and partial characterization of a gene that is inactivated in Escherichia coli. Mol. Cell. Biol. 7: 1180-1192, 1987. PubMed: 3550431 Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151 |
