| 产品名称： | pGEM4Z-HTL3 |
|---|---|
| 商品货号： | TS188179 |
| Designations： | pGEM4Z-HTL3 |
| GenBank Number： | J03810 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 5.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.400000095367432 Tissue: liver Gene product: solute carrier family 2 (facilitated glucose transporter), member 2(glucose transporter 2) GLUT2 Alleles: N1, N2, A1, A1, A2, A2, B1, B1, B2, B2, N1, N2, O2, A1, A1, A2, A2, B1, B1, B2, B2, O2, O2, O2 |
| Insert Size (kb)： | 2.400 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | The insert includes the following restriction sites (nucleotide position according to the published sequence): SacI--451; HindIII--812; XmnI--1208; PstI--1525; EcoRI--1530. Restriction digests of the clone give the following sizes (kb): SalI--2.9, 2.4; EcoRI--3.8, 1.5; HindIII--3.6, 1.7; BamHI--5.3; PstI--4.4, 0.9; consistent with deposited DNA. The insert contains the complete coding sequence, including nucleotides 31-2471 of the published sequence. This probe detects transcripts of 2.8, 3.4, and 5.4 kb, which appear to differ in the lengths of the 3 untranslated regions. HTL-1, which contains a subset of the sequences in this clone, hybridized to human BamHI fragments of 12.0 and 6.7 kb, a mouse BamHI fragment of 15.0 kb, and a human EcoRI fragment of 8.3 kb. The 5 end of the insert is adjacent to the SP6 promoter. |
| References： | Fukumoto H, et al. Sequence, tissue distribution, and chromosomal localization of mRNA encoding a human glucose transporter-like protein. Proc. Natl. Acad. Sci. USA 85: 5434-5438, 1988. PubMed: 3399500 Chappell PB, et al. Polymorphism at the D20S13 (pphi64) locus. Nucleic Acids Res. 18: 4956, 1990. PubMed: 1975666 Horsthemke B, et al. Two EcoRI RFLPs at the GLUT2 locus. Nucleic Acids Res. 18: 6175, 1990. PubMed: 1978290 Froguel P, et al. Two TaqI RFLPs at the GLUT2 locus in French caucasian population. Nucleic Acids Res. 19: 5799, 1991. PubMed: 1682886 Graeme I Bell, personal communication |
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| 产品名称： | pGEM4Z-HTL3 |
|---|---|
| 商品货号： | TS188179 |
| Designations： | pGEM4Z-HTL3 |
| GenBank Number： | J03810 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 5.400000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.400000095367432 Tissue: liver Gene product: solute carrier family 2 (facilitated glucose transporter), member 2(glucose transporter 2) GLUT2 Alleles: N1, N2, A1, A1, A2, A2, B1, B1, B2, B2, N1, N2, O2, A1, A1, A2, A2, B1, B1, B2, B2, O2, O2, O2 |
| Insert Size (kb)： | 2.400 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | The insert includes the following restriction sites (nucleotide position according to the published sequence): SacI--451; HindIII--812; XmnI--1208; PstI--1525; EcoRI--1530. Restriction digests of the clone give the following sizes (kb): SalI--2.9, 2.4; EcoRI--3.8, 1.5; HindIII--3.6, 1.7; BamHI--5.3; PstI--4.4, 0.9; consistent with deposited DNA. The insert contains the complete coding sequence, including nucleotides 31-2471 of the published sequence. This probe detects transcripts of 2.8, 3.4, and 5.4 kb, which appear to differ in the lengths of the 3 untranslated regions. HTL-1, which contains a subset of the sequences in this clone, hybridized to human BamHI fragments of 12.0 and 6.7 kb, a mouse BamHI fragment of 15.0 kb, and a human EcoRI fragment of 8.3 kb. The 5 end of the insert is adjacent to the SP6 promoter. |
| References： | Fukumoto H, et al. Sequence, tissue distribution, and chromosomal localization of mRNA encoding a human glucose transporter-like protein. Proc. Natl. Acad. Sci. USA 85: 5434-5438, 1988. PubMed: 3399500 Chappell PB, et al. Polymorphism at the D20S13 (pphi64) locus. Nucleic Acids Res. 18: 4956, 1990. PubMed: 1975666 Horsthemke B, et al. Two EcoRI RFLPs at the GLUT2 locus. Nucleic Acids Res. 18: 6175, 1990. PubMed: 1978290 Froguel P, et al. Two TaqI RFLPs at the GLUT2 locus in French caucasian population. Nucleic Acids Res. 19: 5799, 1991. PubMed: 1682886 Graeme I Bell, personal communication |
