| 产品名称： | D-155 |
|---|---|
| 商品货号： | TS188844 |
| Designations： | D-155 |
| GenBank Number： | M85168 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 5.039999961853027 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGEM-3Zf(+) Intact vector size: 3.199 Type of vector: phagemid Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII Polylinker sites: T7-EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII-SP6 Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR promoter: lac, SP6, T7 replicon: pMB1, f1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: AGL Genomic copy number: unique Gene name: amylo-1,6-glucosidase, 4-alpha-glucanotransferase (glycogen debranching enzyme, glycogen storage disease type III) Contains complete coding sequence?: U Chromosome: 1; Localization: 1 p21 Tissue: muscle Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 1.842 Cross references: DNA Seq. Acc.: M85168 Insert lengths(kb): 1.842000007629395 Tissue: muscle Gene product: amylo-1,6-glucosidase, 4-alpha-glucanotransferase (glycogen debranching enzyme, glycogen storage disease type III) AGL |
| Insert Size (kb)： | 1.842 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/PstI--3.2, 1.8; EcoRI/HindIII--3.2, 1.3, 0.5; HindIII--3.7, 1.35; SacI--3.7, 1.35; XbaI--4.8, 0.25; NdeI--3.3, 1.0, 0.65. The insert contains the following restriction sites (kb from the 5 end): EcoRI--1.72; HindIII--0.41; NdeI--0.18, 0.86; PvuII--0.75; SacI--0.38; XbaI--1.55. Detects a transcript of approximately 7 kb. |
| References： | Yang BZ, et al. Molecular cloning and nucleotide sequence of cDNA encoding human muscle glycogen debranching enzyme. J. Biol. Chem. 267: 9294-9299, 1992. PubMed: 1374391 Yuan-Tsong Chen, personal communication |
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| 产品名称： | D-155 |
|---|---|
| 商品货号： | TS188844 |
| Designations： | D-155 |
| GenBank Number： | M85168 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 5.039999961853027 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pGEM-3Zf(+) Intact vector size: 3.199 Type of vector: phagemid Cloning sites: EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII Polylinker sites: T7-EcoRI SacI KpnI AvaI SmaI BamHI XbaI SalI AccI HincII PstI SphI HindIII-SP6 Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR promoter: lac, SP6, T7 replicon: pMB1, f1 Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: AGL Genomic copy number: unique Gene name: amylo-1,6-glucosidase, 4-alpha-glucanotransferase (glycogen debranching enzyme, glycogen storage disease type III) Contains complete coding sequence?: U Chromosome: 1; Localization: 1 p21 Tissue: muscle Type of DNA: cDNA Insert end: Insert end: Insert size (kb): 1.842 Cross references: DNA Seq. Acc.: M85168 Insert lengths(kb): 1.842000007629395 Tissue: muscle Gene product: amylo-1,6-glucosidase, 4-alpha-glucanotransferase (glycogen debranching enzyme, glycogen storage disease type III) AGL |
| Insert Size (kb)： | 1.842 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/PstI--3.2, 1.8; EcoRI/HindIII--3.2, 1.3, 0.5; HindIII--3.7, 1.35; SacI--3.7, 1.35; XbaI--4.8, 0.25; NdeI--3.3, 1.0, 0.65. The insert contains the following restriction sites (kb from the 5 end): EcoRI--1.72; HindIII--0.41; NdeI--0.18, 0.86; PvuII--0.75; SacI--0.38; XbaI--1.55. Detects a transcript of approximately 7 kb. |
| References： | Yang BZ, et al. Molecular cloning and nucleotide sequence of cDNA encoding human muscle glycogen debranching enzyme. J. Biol. Chem. 267: 9294-9299, 1992. PubMed: 1374391 Yuan-Tsong Chen, personal communication |
