1G2

规格:

货期:

编号:TS188904

品牌:Testobio

产品名称：	1G2
商品货号：	TS188904
Organism：	Homo sapiens, human
Tissue：	embryonic kidney
Cell Type：	epithelial; somatic cell hybrid
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	2 cells contain Ad5, SV40, and EBV viral DNA sequences Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	Burkitts lymphoma
Applications：	The 1G2 cell line was established by transfection of HKB-11 cells (ATCC CRL-12568) with pSH157 expression vector coding for an IL-4 selective agonist, IL-4SA. This cell line can be used for relatively quick production of small amounts of IL-4SA protein (gram quantities).
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	The 1G2 cell line was established by transfection of HKB-11 cells (ATCC CRL-12568) with pSH157 expression vector coding for an IL-4 selective agonist, IL-4SA. This cell line can be used for relatively quick production of small amounts of IL-4SA protein (gram quantities). The 1G2 cell line was deposited at the ATCC and is available as PTA-87.
Comments：	The 1G2 cell line was established by transfection of HKB-11 cells (ATCC CRL-12568) with pSH157 expression vector coding for an IL-4 selective agonist, IL-4SA. This cell line can be used for relatively quick production of small amounts of IL-4SA protein (gram quantities). The 1G2 cell line was deposited at the ATCC and is available as PTA-87.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Note: Cells will slough off easily, subculture before confuency Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:4 to 1:6 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Name of Depositor：	Bayer Corporation
References：	Cho MS. Human hybrid host cell for mammalian gene expression . US Patent 6,136,599 dated Oct 24 2000 Cho MS, et al. Expression system for factor VIII. US Patent 6,358,703 dated Mar 19 2002 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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1G2

货号: TS188904
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产品名称：	1G2
商品货号：	TS188904
Organism：	Homo sapiens, human
Tissue：	embryonic kidney
Cell Type：	epithelial; somatic cell hybrid
Product Format：	frozen
Morphology：	epithelial
Culture Properties：	adherent
Biosafety Level：	2 cells contain Ad5, SV40, and EBV viral DNA sequences Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	Burkitts lymphoma
Applications：	The 1G2 cell line was established by transfection of HKB-11 cells (ATCC CRL-12568) with pSH157 expression vector coding for an IL-4 selective agonist, IL-4SA. This cell line can be used for relatively quick production of small amounts of IL-4SA protein (gram quantities).
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	The 1G2 cell line was established by transfection of HKB-11 cells (ATCC CRL-12568) with pSH157 expression vector coding for an IL-4 selective agonist, IL-4SA. This cell line can be used for relatively quick production of small amounts of IL-4SA protein (gram quantities). The 1G2 cell line was deposited at the ATCC and is available as PTA-87.
Comments：	The 1G2 cell line was established by transfection of HKB-11 cells (ATCC CRL-12568) with pSH157 expression vector coding for an IL-4 selective agonist, IL-4SA. This cell line can be used for relatively quick production of small amounts of IL-4SA protein (gram quantities). The 1G2 cell line was deposited at the ATCC and is available as PTA-87.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
Subculturing：	Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Note: Cells will slough off easily, subculture before confuency Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:4 to 1:6 Medium Renewal: Every 2 to 3 days Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation：	Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions：	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
Name of Depositor：	Bayer Corporation
References：	Cho MS. Human hybrid host cell for mammalian gene expression . US Patent 6,136,599 dated Oct 24 2000 Cho MS, et al. Expression system for factor VIII. US Patent 6,358,703 dated Mar 19 2002 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.