| 产品名称： | pCMV5FIX |
|---|---|
| 商品货号： | TS189239 |
| Designations： | pCMV5FIX |
| GenBank Number： | J00136 |
| Species： | Homo sapiens, human |
| Applications： | in another host, produces protein coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) |
| Vector： | Construct size (kb): 7.400000095367432 |
| Insert： | DNA: cDNA Insert information: Insert size (kb): 2.8 Genexa0: F9, coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) DNAxa0: cDNA Sourcexa0: Human Genbank accessionxa0: J00136 Insert endsxa0: BamHI, XbaI Insert lengths(kb): 2.799999952316284 Gene product: coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) F9 Alleles: A1, A2, D2, C1, D1, C2 Target Gene: coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) |
| Insert Size (kb)： | 2.800 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/BglII--4.6, 2.9; BamHI--7.6; BglII--7.6; EcoRI--4.9, 2.7. Digestion with EcoRI produces fragments of 4.9 and 2.5 kb. Bacteria may grow slowly due to the high plasmid copy number. Corresponds to the wild-type sequence. |
| References： | Andersson S, et al. Cloning, structure, and expression of a mitochondrial cytochrome P-450 sterol 26-hyroxylase, a bile acid biosnythetic enzyme. J. Biol. Chem. 264: 8222-8229, 1989. PubMed: 2722778 Cheung WF, et al. The binding of human factor IX to endothelial cells is mediated by residues 3-11. J. Biol. Chem. 267: 20529-20531, 1992. PubMed: 1400370 Cheung WF, et al. The role of the epidermal growth factor-1 and hydrophobic stack domains of human factor IX in binding to endothelial cells. J. Biol. Chem. 266: 8797-8800, 1991. PubMed: 1851159 Wing-Fai Cheung, personal communication |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pCMV5FIX |
|---|---|
| 商品货号： | TS189239 |
| Designations： | pCMV5FIX |
| GenBank Number： | J00136 |
| Species： | Homo sapiens, human |
| Applications： | in another host, produces protein coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) |
| Vector： | Construct size (kb): 7.400000095367432 |
| Insert： | DNA: cDNA Insert information: Insert size (kb): 2.8 Genexa0: F9, coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) DNAxa0: cDNA Sourcexa0: Human Genbank accessionxa0: J00136 Insert endsxa0: BamHI, XbaI Insert lengths(kb): 2.799999952316284 Gene product: coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) F9 Alleles: A1, A2, D2, C1, D1, C2 Target Gene: coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) |
| Insert Size (kb)： | 2.800 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/BglII--4.6, 2.9; BamHI--7.6; BglII--7.6; EcoRI--4.9, 2.7. Digestion with EcoRI produces fragments of 4.9 and 2.5 kb. Bacteria may grow slowly due to the high plasmid copy number. Corresponds to the wild-type sequence. |
| References： | Andersson S, et al. Cloning, structure, and expression of a mitochondrial cytochrome P-450 sterol 26-hyroxylase, a bile acid biosnythetic enzyme. J. Biol. Chem. 264: 8222-8229, 1989. PubMed: 2722778 Cheung WF, et al. The binding of human factor IX to endothelial cells is mediated by residues 3-11. J. Biol. Chem. 267: 20529-20531, 1992. PubMed: 1400370 Cheung WF, et al. The role of the epidermal growth factor-1 and hydrophobic stack domains of human factor IX in binding to endothelial cells. J. Biol. Chem. 266: 8797-8800, 1991. PubMed: 1851159 Wing-Fai Cheung, personal communication |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
