| 产品名称： | pFV17 |
|---|---|
| 商品货号： | TS189257 |
| Designations： | pFV17 |
| Species： | Saccharomyces cerevisiae, 2 micron circle |
| Depositors： | JR Broach |
| Applications： | in another host, produces protein recombinase, site-specific |
| Vector： | Construct size (kb): 6.800000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Saccharomyces cerevisiae, 2 micron circle Gene symbol: FLP Genomic copy number: unique Gene name: recombinase, site-specific Contains complete coding sequence?: U Type of DNA: genomic Insert end: Insert end: Insert size (kb): Cross references: Gene product: recombinase, site-specific FLP Target Gene: recombinase, site-specific |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.8; EcoRI--3.4, 2.15, 1.25; SalI--6.8; SalI/HindIII--5.9, 0.9; SalI/XbaI--5.3, 1.5. Linearize with BstEII before transformation into S. cerevisiae hosts. Integrating plasmid for galactose-inducible expression of FLP (from the GAL10 promoter). Constructed by cloning the HaeIII/HindIII fragment from the 2 micron circle into the SalI/HindIII sites of YEp51 (using a SalI linker), modifying the region between GAL10 and FLP to increase expression, and deleting the 2 micron origin of replication. Derived from pBR322 via YEp51. |
| References： | Rose AB, Broach JR. Propagation and expression of cloned genes in yeast: 2-micron circle-based vectors. Methods Enzymol. 185: 234-279, 1990. PubMed: 2199781 Volkert FC, Broach JR. Site-specific recombination promotes plasmid amplification in yeast. Cell 46: 541-550, 1986. PubMed: 3524855 |
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| 产品名称： | pFV17 |
|---|---|
| 商品货号： | TS189257 |
| Designations： | pFV17 |
| Species： | Saccharomyces cerevisiae, 2 micron circle |
| Depositors： | JR Broach |
| Applications： | in another host, produces protein recombinase, site-specific |
| Vector： | Construct size (kb): 6.800000190734863 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Saccharomyces cerevisiae, 2 micron circle Gene symbol: FLP Genomic copy number: unique Gene name: recombinase, site-specific Contains complete coding sequence?: U Type of DNA: genomic Insert end: Insert end: Insert size (kb): Cross references: Gene product: recombinase, site-specific FLP Target Gene: recombinase, site-specific |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--6.8; EcoRI--3.4, 2.15, 1.25; SalI--6.8; SalI/HindIII--5.9, 0.9; SalI/XbaI--5.3, 1.5. Linearize with BstEII before transformation into S. cerevisiae hosts. Integrating plasmid for galactose-inducible expression of FLP (from the GAL10 promoter). Constructed by cloning the HaeIII/HindIII fragment from the 2 micron circle into the SalI/HindIII sites of YEp51 (using a SalI linker), modifying the region between GAL10 and FLP to increase expression, and deleting the 2 micron origin of replication. Derived from pBR322 via YEp51. |
| References： | Rose AB, Broach JR. Propagation and expression of cloned genes in yeast: 2-micron circle-based vectors. Methods Enzymol. 185: 234-279, 1990. PubMed: 2199781 Volkert FC, Broach JR. Site-specific recombination promotes plasmid amplification in yeast. Cell 46: 541-550, 1986. PubMed: 3524855 |
