| 产品名称： | H2-10 pH2-10 |
|---|---|
| 商品货号： | TS189430 |
| Designations： | H2-10 pH2-10 |
| Species： | Homo sapiens, human |
| Depositors： | SA Latt |
| Applications： | HgiAI also reveals a 2-allele polymorphism (5.2 kb, 4.3 kb) but this has not been verified by pedigree studies. |
| Vector： | Construct size (kb): 5.699999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 1.299999952316284 Gene product: DNA Segment, single copy D13S26 Alleles: B2, C1, D1, D2, A1, A2, B1, C2 |
| Insert Size (kb)： | 1.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--4.4, 1.3; EcoRI--5.7; EcoRI/HindIII--4.4, 1.3; SalI--5.7. The EcoRI and BclI polymorphisms appear to be due to the same event. The BclI, HphI, and Bsp1286 polymorphisms are occasionally different. HgiAI also reveals a 2-allele polymorphism (5.2 kb, 4.3 kb) but this has not been verified by pedigree studies. Enzyme(s) not detecting polymorphism in a panel of 6 unrelated Caucasians: AccI, ApaLI, BglII, BstNI, EcoRV, HaeIII, HincII, HindIII, KpnI, MboI, PstI, PvuII, RsaI, Sau96I, ScaI, ScrfI, SstI, StyI, TaqI, XmnI. |
| References： | Lalande M, et al. Molecular detection and differentiation of deletions in band 13q14 in human retinoblastoma. Cancer Genet. Cytogenet. 23: 151-157, 1986. PubMed: 3756834 Lalande M, et al. Isolation of human chromosome 13-specific DNA sequences cloned from flow sorted chromosomes and potentially linked to the retinoblastoma locus. Cancer Genet. Cytogenet. 13: 283-295, 1984. PubMed: 6210139 Bowcock AM, et al. Polymorphisms revealed by random probe H2-10 D13S26 which maps to chromosome 13q21-q22. Nucleic Acids Res. 16: 2745, 1988. PubMed: 2896341 |
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| 产品名称： | H2-10 pH2-10 |
|---|---|
| 商品货号： | TS189430 |
| Designations： | H2-10 pH2-10 |
| Species： | Homo sapiens, human |
| Depositors： | SA Latt |
| Applications： | HgiAI also reveals a 2-allele polymorphism (5.2 kb, 4.3 kb) but this has not been verified by pedigree studies. |
| Vector： | Construct size (kb): 5.699999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 1.299999952316284 Gene product: DNA Segment, single copy D13S26 Alleles: B2, C1, D1, D2, A1, A2, B1, C2 |
| Insert Size (kb)： | 1.300 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--4.4, 1.3; EcoRI--5.7; EcoRI/HindIII--4.4, 1.3; SalI--5.7. The EcoRI and BclI polymorphisms appear to be due to the same event. The BclI, HphI, and Bsp1286 polymorphisms are occasionally different. HgiAI also reveals a 2-allele polymorphism (5.2 kb, 4.3 kb) but this has not been verified by pedigree studies. Enzyme(s) not detecting polymorphism in a panel of 6 unrelated Caucasians: AccI, ApaLI, BglII, BstNI, EcoRV, HaeIII, HincII, HindIII, KpnI, MboI, PstI, PvuII, RsaI, Sau96I, ScaI, ScrfI, SstI, StyI, TaqI, XmnI. |
| References： | Lalande M, et al. Molecular detection and differentiation of deletions in band 13q14 in human retinoblastoma. Cancer Genet. Cytogenet. 23: 151-157, 1986. PubMed: 3756834 Lalande M, et al. Isolation of human chromosome 13-specific DNA sequences cloned from flow sorted chromosomes and potentially linked to the retinoblastoma locus. Cancer Genet. Cytogenet. 13: 283-295, 1984. PubMed: 6210139 Bowcock AM, et al. Polymorphisms revealed by random probe H2-10 D13S26 which maps to chromosome 13q21-q22. Nucleic Acids Res. 16: 2745, 1988. PubMed: 2896341 |
