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Naegleria lovaniensis Steven et al.
Naegleria lovaniensis Steven et al.
规格:
货期:
编号:TS189648
品牌:Testobio
产品名称： Naegleria lovaniensis Steven et al.
商品货号： TS189648
Deposited As： Naegleria gruberi Schardinger
Strain Designations： TS
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： Vero cell culture 120th passage, Bowling Green, OH, 1970
Product Format： frozen
Storage Conditions： Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain： no
Comments：
Nutritional study of Naegleria gruberi
Medium： ATCC® Medium 1034: Modified PYNFH medium (Available from ATCC as ATCC cat. no. 327-X)
ATCC® Medium 935: NYTG Medium
ATCC® Medium 710: Nelsons Culture Medium For Naegleria
ATCC® Medium 803: M7 medium
ATCC® Medium 902: Schusters axenic Naegleria medium
Growth Conditions：
Temperature: 25°C
Culture System: Axenic
Cryopreservation： Harvest and Preservation
  1. Harvest cells from a culture that is at or near peak density by centrifugation at 600 x g for 5 min. Pool the cell pellets into a single tube.
  2. Adjust the concentration of cells to 2.0 x 106/mL.xa0 If the concentration is too low, centrifuge at 600 x g for 5 minutes and resuspend the cell pellet with a volume of supernatant to yield the desired concentration.
  3. Prepare a 15% (v/v) sterile DMSO solution in ATCC medium 1034 as follows:xa0 Add the required volume of DMSO to a glass screw-capped test tube and place on ice.xa0 Allow the DMSO to solidify.xa0 Add the required volume of refrigerated ATCC medium 1034.xa0 Dissolve the DMSO by inverting several times.xa0 If the DMSO solution is not prepared on ice, an exothermic reaction will occur that may precipitate certain components of the medium.
  4. Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 106 and 7.5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution to the start of the freezing process should be no less than 15 min and no longer than 60 min.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen.
  7. The frozen preparations are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just sufficiently to cover only the frozen material. Do not agitate the vial.
  9. Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate into a fresh tube or flask of ATCC medium 1034.
  10. Incubate at 25°C with the cap screwed on tightly (incubate a test tube on a 15° horizontal slant).
Name of Depositor： WD ODell
Year of Origin： 1970
References：

ODell WD, Brent MM. Nutritional study of three strains of Naegleria gruberi. J. Protozool. 21: 129-133, 1974. PubMed: 4206403

ODell WD, Stevens AR. Quantitative growth of Naegleria in axenic culture. Appl. Microbiol. 25: 621-627, 1973. PubMed: 4633479

De Jonckheere JF. A century of research on the amoeboflagellate genus Naegleria. Acta Protozool. 41: 309-342, 2002.

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Naegleria lovaniensis Steven et al.

  • 货号: TS189648
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称： Naegleria lovaniensis Steven et al.
商品货号： TS189648
Deposited As： Naegleria gruberi Schardinger
Strain Designations： TS
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： Vero cell culture 120th passage, Bowling Green, OH, 1970
Product Format： frozen
Storage Conditions： Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain： no
Comments：
Nutritional study of Naegleria gruberi
Medium： ATCC® Medium 1034: Modified PYNFH medium (Available from ATCC as ATCC cat. no. 327-X)
ATCC® Medium 935: NYTG Medium
ATCC® Medium 710: Nelsons Culture Medium For Naegleria
ATCC® Medium 803: M7 medium
ATCC® Medium 902: Schusters axenic Naegleria medium
Growth Conditions：
Temperature: 25°C
Culture System: Axenic
Cryopreservation： Harvest and Preservation
  1. Harvest cells from a culture that is at or near peak density by centrifugation at 600 x g for 5 min. Pool the cell pellets into a single tube.
  2. Adjust the concentration of cells to 2.0 x 106/mL.xa0 If the concentration is too low, centrifuge at 600 x g for 5 minutes and resuspend the cell pellet with a volume of supernatant to yield the desired concentration.
  3. Prepare a 15% (v/v) sterile DMSO solution in ATCC medium 1034 as follows:xa0 Add the required volume of DMSO to a glass screw-capped test tube and place on ice.xa0 Allow the DMSO to solidify.xa0 Add the required volume of refrigerated ATCC medium 1034.xa0 Dissolve the DMSO by inverting several times.xa0 If the DMSO solution is not prepared on ice, an exothermic reaction will occur that may precipitate certain components of the medium.
  4. Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 106 and 7.5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution to the start of the freezing process should be no less than 15 min and no longer than 60 min.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion. At -40°C plunge ampules into liquid nitrogen.
  7. The frozen preparations are stored in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just sufficiently to cover only the frozen material. Do not agitate the vial.
  9. Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate into a fresh tube or flask of ATCC medium 1034.
  10. Incubate at 25°C with the cap screwed on tightly (incubate a test tube on a 15° horizontal slant).
Name of Depositor： WD ODell
Year of Origin： 1970
References：

ODell WD, Brent MM. Nutritional study of three strains of Naegleria gruberi. J. Protozool. 21: 129-133, 1974. PubMed: 4206403

ODell WD, Stevens AR. Quantitative growth of Naegleria in axenic culture. Appl. Microbiol. 25: 621-627, 1973. PubMed: 4633479

De Jonckheere JF. A century of research on the amoeboflagellate genus Naegleria. Acta Protozool. 41: 309-342, 2002.

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