| 产品名称： | pHIL6R.3 |
|---|---|
| 商品货号： | TS189671 |
| Designations： | pHIL6R.3 |
| GenBank Number： | M20566 |
| Species： | Homo sapiens, human |
| Depositors： | GH Fey |
| Applications： | The sequence was confirmed by partial sequence analysis and it includes and matches nucleotides 250 to 3347 of the published sequence. A 1.209 kb SphI fragment of the probe was used to detect a genomic PvuII fragment of 7.1 kb in the rat and a genomic PvuII fragment of 3.7 kb in the mouse. |
| Vector： | Construct size (kb): 6.060999870300293 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.096999883651733 Tissue: HL-60 cell line (promyelocytic), differentiated Gene product: interleukin 6 receptor IL6R |
| Insert Size (kb)： | 3.097 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | The insert includes the following restriction sites (in nucleotides from the 5 end of the published sequence): SmaI--319, 580; SphI--440, 1649; PstI--638, 2780, 3256; SacI--1201; XhoI--1763. Restriction digests of the clone give the following sizes (kb): EcoRI--3.3, 3.0; HindIII--5.1, 1.35; BamHI--6.4; PstI--3.6, 2.2, 0.59; SalI--4.1, 2.2. The sequence was confirmed by partial sequence analysis and it includes and matches nucleotides 250 to 3347 of the published sequence. It encodes the complete coding region (437 - 1841) plus some 5 and 3 untranslated regions. A 1.209 kb SphI fragment of the probe was used to detect a genomic PvuII fragment of 7.1 kb in the rat and a genomic PvuII fragment of 3.7 kb in the mouse. The insert is oriented such that linearizing with BamHI and using the T3 promoter gives the sense strand, and linearizing with EcoRI and using the T7 promoter gives the antisense strand. |
| References： | Baumann M, et al. Molecular cloning, characterization and functional expression of the rat liver interleukin 6 receptor. J. Biol. Chem. 265: 19853-19862, 1990. PubMed: 2174054 Yamasaki K, et al. Cloning and expression of the human interleukin-6 (BSF-2/IFN-beta2) receptor. Science 241: 825-828, 1988. PubMed: 3136546 Szpirer J, et al. The Interleukin-6-dependent DNA-binding protein gene (transcription factor 5: TCF5) maps to human chromosome 20 and rat chromosome 3, the IL6 receptor locus (IL6R) to human chromosome 1 and rat chromosome 2, and the rat IL6 gene to rat chromosome 4. Genomics 10: 539-546, 1991. PubMed: 1889804 Georg H Fey, personal communication |
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| 产品名称： | pHIL6R.3 |
|---|---|
| 商品货号： | TS189671 |
| Designations： | pHIL6R.3 |
| GenBank Number： | M20566 |
| Species： | Homo sapiens, human |
| Depositors： | GH Fey |
| Applications： | The sequence was confirmed by partial sequence analysis and it includes and matches nucleotides 250 to 3347 of the published sequence. A 1.209 kb SphI fragment of the probe was used to detect a genomic PvuII fragment of 7.1 kb in the rat and a genomic PvuII fragment of 3.7 kb in the mouse. |
| Vector： | Construct size (kb): 6.060999870300293 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.096999883651733 Tissue: HL-60 cell line (promyelocytic), differentiated Gene product: interleukin 6 receptor IL6R |
| Insert Size (kb)： | 3.097 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | The insert includes the following restriction sites (in nucleotides from the 5 end of the published sequence): SmaI--319, 580; SphI--440, 1649; PstI--638, 2780, 3256; SacI--1201; XhoI--1763. Restriction digests of the clone give the following sizes (kb): EcoRI--3.3, 3.0; HindIII--5.1, 1.35; BamHI--6.4; PstI--3.6, 2.2, 0.59; SalI--4.1, 2.2. The sequence was confirmed by partial sequence analysis and it includes and matches nucleotides 250 to 3347 of the published sequence. It encodes the complete coding region (437 - 1841) plus some 5 and 3 untranslated regions. A 1.209 kb SphI fragment of the probe was used to detect a genomic PvuII fragment of 7.1 kb in the rat and a genomic PvuII fragment of 3.7 kb in the mouse. The insert is oriented such that linearizing with BamHI and using the T3 promoter gives the sense strand, and linearizing with EcoRI and using the T7 promoter gives the antisense strand. |
| References： | Baumann M, et al. Molecular cloning, characterization and functional expression of the rat liver interleukin 6 receptor. J. Biol. Chem. 265: 19853-19862, 1990. PubMed: 2174054 Yamasaki K, et al. Cloning and expression of the human interleukin-6 (BSF-2/IFN-beta2) receptor. Science 241: 825-828, 1988. PubMed: 3136546 Szpirer J, et al. The Interleukin-6-dependent DNA-binding protein gene (transcription factor 5: TCF5) maps to human chromosome 20 and rat chromosome 3, the IL6 receptor locus (IL6R) to human chromosome 1 and rat chromosome 2, and the rat IL6 gene to rat chromosome 4. Genomics 10: 539-546, 1991. PubMed: 1889804 Georg H Fey, personal communication |
