| 产品名称： | cMCOE32 |
|---|---|
| 商品货号： | TS190216 |
| Designations： | cMCOE32 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Applications： | Codominant segregation of the MspI RFLP was observed in 54 three-generation families. RFLPs were observed under total human DNA prehybridization and hybridization. |
| Vector： | Construct size (kb): 45.0 |
| Insert： | DNA: genomic Insert lengths(kb): 38.0 Gene product: DNA Segment, single copy D2S53 |
| Insert Size (kb)： | 38.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--12.5, 9.6, 9.4, 7.9, 6.6; HindIII--15.5, 10.0, 7.2, 3.5, 3.2, 2.6, 2.3, 1.9; BglII--25, 20; SalI--25, 10.5, 9.1; SfiI--21, 15, 9.0; EcoRI/HindIII--11.5, 6.8 (doublet), 4.0, 3.8, 3.3, 2.8, 2.0, 1.8, 1.2; consistent with deposited DNA. Codominant segregation of the MspI RFLP was observed in 54 three-generation families. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. RFLPs were observed under total human DNA prehybridization and hybridization. |
| References： | Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (cMCOE32) on chromosome 2 D2S53. Nucleic Acids Res. 16: 6258, 1988. PubMed: 2899880 Nakamura Y, et al. New approach for isolation of VNTR markers. Am. J. Hum. Genet. 43: 854-859, 1988. PubMed: 2904220 |
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| 产品名称： | cMCOE32 |
|---|---|
| 商品货号： | TS190216 |
| Designations： | cMCOE32 |
| Species： | Homo sapiens, human |
| Depositors： | RL White, Y Nakamura |
| Applications： | Codominant segregation of the MspI RFLP was observed in 54 three-generation families. RFLPs were observed under total human DNA prehybridization and hybridization. |
| Vector： | Construct size (kb): 45.0 |
| Insert： | DNA: genomic Insert lengths(kb): 38.0 Gene product: DNA Segment, single copy D2S53 |
| Insert Size (kb)： | 38.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--12.5, 9.6, 9.4, 7.9, 6.6; HindIII--15.5, 10.0, 7.2, 3.5, 3.2, 2.6, 2.3, 1.9; BglII--25, 20; SalI--25, 10.5, 9.1; SfiI--21, 15, 9.0; EcoRI/HindIII--11.5, 6.8 (doublet), 4.0, 3.8, 3.3, 2.8, 2.0, 1.8, 1.2; consistent with deposited DNA. Codominant segregation of the MspI RFLP was observed in 54 three-generation families. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. RFLPs were observed under total human DNA prehybridization and hybridization. |
| References： | Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (cMCOE32) on chromosome 2 D2S53. Nucleic Acids Res. 16: 6258, 1988. PubMed: 2899880 Nakamura Y, et al. New approach for isolation of VNTR markers. Am. J. Hum. Genet. 43: 854-859, 1988. PubMed: 2904220 |
