| 产品名称： | p300 |
|---|---|
| 商品货号： | TS190377 |
| Designations： | p300 |
| Species： | Homo sapiens, human |
| Depositors： | W Stetler-Stevenson |
| Vector： | Construct size (kb): 3.263000011444092 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: BamHI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: MMP2 Other gene symbols: CLG4A; CLG4 Gene name: matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase) Contains complete coding sequence?: U Chromosome: 16; Localization: 16 q12.2 Tissue: placenta Type of DNA: cDNA Insert 5 end: EcoRI Insert 3 end: BamHI Insert size (kb): 0.305 Cross references: Insert lengths(kb): 0.3050000071525574 Tissue: placenta Gene product: matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase)(collagenase IVA (basement membrane), metalloproteinase stromelysin-2) CLG4 |
| Insert Size (kb)： | 0.305 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): KpnI--3.1, 0.2; BglI--1.85, 1.20, 0.25; XbaI--3.3; HindIII--3.3; BamHI/EcoRI--3.0, 0.3. The insert contains the following restriction sites (bp from the 5 end): BglI--24; HaeIII--27, 40, 51, 88; KpnI--168; MboI--134; NcoI--20; SacI--59. The insert corresponds to nt 1448-1747 of the published sequence. Corresponds to nt 1730-2030 of the near full length cDNA clone p16SPT19-1. Detects a transcript of 3.1 kb. |
| References： | Levy AT, et al. Increased expression of the Mr 72,000 type IV collagenase in human colonic adenocarcinoma. Cancer Res. 51: 439-444, 1991. PubMed: 1846313 Collier IE, et al. H-ras oncogene-transformed human bronchial epithelial cells (tbe-1)secrete a single metalloprotease capable of degrading basement membrane collagen. J. Biol. Chem. 263: 6579-6587, 1988. PubMed: 2834383 William Stetler-Stevenson, personal communication |
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| 产品名称： | p300 |
|---|---|
| 商品货号： | TS190377 |
| Designations： | p300 |
| Species： | Homo sapiens, human |
| Depositors： | W Stetler-Stevenson |
| Vector： | Construct size (kb): 3.263000011444092 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: BamHI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: MMP2 Other gene symbols: CLG4A; CLG4 Gene name: matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase) Contains complete coding sequence?: U Chromosome: 16; Localization: 16 q12.2 Tissue: placenta Type of DNA: cDNA Insert 5 end: EcoRI Insert 3 end: BamHI Insert size (kb): 0.305 Cross references: Insert lengths(kb): 0.3050000071525574 Tissue: placenta Gene product: matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase)(collagenase IVA (basement membrane), metalloproteinase stromelysin-2) CLG4 |
| Insert Size (kb)： | 0.305 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): KpnI--3.1, 0.2; BglI--1.85, 1.20, 0.25; XbaI--3.3; HindIII--3.3; BamHI/EcoRI--3.0, 0.3. The insert contains the following restriction sites (bp from the 5 end): BglI--24; HaeIII--27, 40, 51, 88; KpnI--168; MboI--134; NcoI--20; SacI--59. The insert corresponds to nt 1448-1747 of the published sequence. Corresponds to nt 1730-2030 of the near full length cDNA clone p16SPT19-1. Detects a transcript of 3.1 kb. |
| References： | Levy AT, et al. Increased expression of the Mr 72,000 type IV collagenase in human colonic adenocarcinoma. Cancer Res. 51: 439-444, 1991. PubMed: 1846313 Collier IE, et al. H-ras oncogene-transformed human bronchial epithelial cells (tbe-1)secrete a single metalloprotease capable of degrading basement membrane collagen. J. Biol. Chem. 263: 6579-6587, 1988. PubMed: 2834383 William Stetler-Stevenson, personal communication |
