Fugu fry

规格:

货期:

编号:TS190609

品牌:Testobio

产品名称：	Fugu fry
商品货号：	TS190609
Organism：	Fugu niphobles, kusafugu
Tissue：	fry, whole
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	1 to 6 days posthatching juvenile
Applications：	The Fugu fry cell line was established in 1996 from normal juvenile whole fry. The cell line may be used for in vitro vertebrate genome studies, and in marine fish and aquaculture research.
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	hyperploid
Derivation：	The Fugu fry cell line was established in 1996 from normal juvenile whole fry.
Comments：	The Fugu fry cell line was established in 1996 from normal juvenile whole fry. Fugu cells maintain an unusually compact genome size with small introns. They are telomerase positive. The cell line may be used for in vitro vertebrate genome studies, and in marine fish and aquaculture research.
Complete Growth Medium：	These cells are grown in 67.5% DMEM HG without sodium bicarbonate (Invitrogen Cat no. 12100) 25% L-15 (ATCC Cat no.30-2008) 7.5% Ham?s F12 without sodium bicarbonate (Invitrogen Cat no. 21700) Supplemented with: 0.5 g/L sodium bicarbonate 15 mM HEPES 0.01 mg/ml bovine insulin 0.05 mM 2-mercaptoethanol 1.0 mM L-glutamine 0.05 mM non-essential amino acids 10 ng/ml basic human recombinant FGF 50 ng/ml mouse EGF (Do not filter) 5 to 7.5% heat-inactivated fetal bovine serum
Subculturing：	Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 21 to 23°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. Incubate cultures at 21 to 23°C without CO2. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended; cell density must be high. Medium Renewal: Every 2 to 3 days
Cryopreservation：	Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 22.0°C; (Max. 23.0 °C, Min. 21.0°C)
Population Doubling Time：	2.5 days
Name of Depositor：	DW Barnes
Deposited As：	Fugu niphobles
Year of Origin：	1996
References：	Bradford CS, et al. Characterization of cell cultures derived from Fugu, the Japanese pufferfish. Mol. Mar. Biol. Biotechnol. 6: 279-288, 1997. PubMed: 9418286

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Fugu fry

货号: TS190609
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产品名称：	Fugu fry
商品货号：	TS190609
Organism：	Fugu niphobles, kusafugu
Tissue：	fry, whole
Product Format：	frozen
Morphology：	fibroblast
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	normal
Age：	1 to 6 days posthatching juvenile
Applications：	The Fugu fry cell line was established in 1996 from normal juvenile whole fry. The cell line may be used for in vitro vertebrate genome studies, and in marine fish and aquaculture research.
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	hyperploid
Derivation：	The Fugu fry cell line was established in 1996 from normal juvenile whole fry.
Comments：	The Fugu fry cell line was established in 1996 from normal juvenile whole fry. Fugu cells maintain an unusually compact genome size with small introns. They are telomerase positive. The cell line may be used for in vitro vertebrate genome studies, and in marine fish and aquaculture research.
Complete Growth Medium：	These cells are grown in 67.5% DMEM HG without sodium bicarbonate (Invitrogen Cat no. 12100) 25% L-15 (ATCC Cat no.30-2008) 7.5% Ham?s F12 without sodium bicarbonate (Invitrogen Cat no. 21700) Supplemented with: 0.5 g/L sodium bicarbonate 15 mM HEPES 0.01 mg/ml bovine insulin 0.05 mM 2-mercaptoethanol 1.0 mM L-glutamine 0.05 mM non-essential amino acids 10 ng/ml basic human recombinant FGF 50 ng/ml mouse EGF (Do not filter) 5 to 7.5% heat-inactivated fetal bovine serum
Subculturing：	Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 21 to 23°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. Incubate cultures at 21 to 23°C without CO2. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended; cell density must be high. Medium Renewal: Every 2 to 3 days
Cryopreservation：	Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 22.0°C; (Max. 23.0 °C, Min. 21.0°C)
Population Doubling Time：	2.5 days
Name of Depositor：	DW Barnes
Deposited As：	Fugu niphobles
Year of Origin：	1996
References：	Bradford CS, et al. Characterization of cell cultures derived from Fugu, the Japanese pufferfish. Mol. Mar. Biol. Biotechnol. 6: 279-288, 1997. PubMed: 9418286