pRS411
pRS411
规格:
货期:
编号:TS190730
品牌:Testobio
| 产品名称： | pRS411 |
|---|---|
| 商品货号： | TS190730 |
| Designations： | pRS411 |
| Depositors： | JD Boeke |
| Biosafety Level： | 1 |
| Host： | Escherichia coli HB101 (ATCC® 33694™) |
| Vector Information： | Intact vector size: 5.407
Type of vector: phagemid
Cloning sites: SacI BstXI SacII EagI NotI SpeI BamHI SmaI PstI ClaI SalIxa0XhoI ApaI KpnI Polylinker sites: SacI BstXI SacII EagI NotI XbaI SpeI BamHI SmaI PstI EcoRIxa0EcoRV ClaI SalI XhoI ApaI KpnI Construction: pRS401, pRS84 Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): MET15, →, 421-1270 replicon: f1, ←, 1860-2316 promoter for in vitro transcription: T7, →, 2486-2505 MCS: KpnI...SacI, →, 2513-2615 promoter for in vitro transcription: T3, ←, 2632-2651 coding sequence: lacZ, ←, 2317-2676 promoter: lac, ←, 2721-2749 replicon: pMB1, 3075-3075 marker(s): ampR, ←, 3833-4693 centromere: CEN6, 4835-4951 replicon: ARS4, 4952-5353 Vector: pRS411 (phagemid) |
| Applications： | YC-type (centromeric) shuttle vectorxa0
vector permitting visual detection of recombinants O-acetylhomoserine sulfhydrylase |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.4; EcoRI--3.7, 1.7; XbaI--3.4, 2.0. met15 phenotype produces brown colonies when grown on Pb containing media. MET15, MET17 and MET25 are synonymous. This vector is useful for gene knockout experiments in hosts with a non-revertable met15 auxotrophic marker gene mutation. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Cost GJ, Boeke JD. A useful colony colour phenotype associated with the yeast selectable/counter-selectable marker MET15. Yeast 12: 939-941, 1996. PubMed: 8873447 Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801 |
| Related Products： | Component of: ATCC 87476
Component of: ATCC 87529 |
| Shipped： | frozen |
| Shipping Information： | Plasmid in E. coli |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
pRS411
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | pRS411 |
|---|---|
| 商品货号： | TS190730 |
| Designations： | pRS411 |
| Depositors： | JD Boeke |
| Biosafety Level： | 1 |
| Host： | Escherichia coli HB101 (ATCC® 33694™) |
| Vector Information： | Intact vector size: 5.407
Type of vector: phagemid
Cloning sites: SacI BstXI SacII EagI NotI SpeI BamHI SmaI PstI ClaI SalIxa0XhoI ApaI KpnI Polylinker sites: SacI BstXI SacII EagI NotI XbaI SpeI BamHI SmaI PstI EcoRIxa0EcoRV ClaI SalI XhoI ApaI KpnI Construction: pRS401, pRS84 Host range: Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): marker(s): MET15, →, 421-1270 replicon: f1, ←, 1860-2316 promoter for in vitro transcription: T7, →, 2486-2505 MCS: KpnI...SacI, →, 2513-2615 promoter for in vitro transcription: T3, ←, 2632-2651 coding sequence: lacZ, ←, 2317-2676 promoter: lac, ←, 2721-2749 replicon: pMB1, 3075-3075 marker(s): ampR, ←, 3833-4693 centromere: CEN6, 4835-4951 replicon: ARS4, 4952-5353 Vector: pRS411 (phagemid) |
| Applications： | YC-type (centromeric) shuttle vectorxa0
vector permitting visual detection of recombinants O-acetylhomoserine sulfhydrylase |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.4; EcoRI--3.7, 1.7; XbaI--3.4, 2.0. met15 phenotype produces brown colonies when grown on Pb containing media. MET15, MET17 and MET25 are synonymous. This vector is useful for gene knockout experiments in hosts with a non-revertable met15 auxotrophic marker gene mutation. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Cost GJ, Boeke JD. A useful colony colour phenotype associated with the yeast selectable/counter-selectable marker MET15. Yeast 12: 939-941, 1996. PubMed: 8873447 Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801 |
| Related Products： | Component of: ATCC 87476
Component of: ATCC 87529 |
| Shipped： | frozen |
| Shipping Information： | Plasmid in E. coli |
