| 产品名称： | hE61.f1 |
|---|---|
| 商品货号： | TS190810 |
| Designations： | hE61.f1 |
| GenBank Number： | U18991 |
| Species： | Homo sapiens, human |
| Depositors： | DA Thompson |
| Vector： | Construct size (kb): 5.599999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.599999904632568 Tissue: retinal pigment epithelium Gene product: retinal pigment epithelium-specific protein (65kD) RPE65 |
| Insert Size (kb)： | 2.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 2.7; SmaI--4.4, 1.0; PstI--2.9, 1.8, 0.65. The 2.6kb insert differs from the GenBank entry as follows: A17C, G35C, G36A, G234A, G2573C, T2601C and it contains 24 additional nucleotides at the 3 end. The 24 additional nucleotides (GGG CTG CAG GAA TTC CTG CAG GGG) introduce EcoRI, SmaI, PstI restriction sites at the 3 end of the insert. Inserts detects an insertion/deletion polymorphism at the following nucleotides of the GenBank record G2384del and A2385del. |
| References： | Nicoletti A, et al. Molecular characterization of the human gene encoding abundant 61 kDA protein specific to the retinal pigment epithelium. Hum. Mol. Genet. 4: 641-649, 1995. PubMed: 7633413 Gu SM, et al. Mutations in RPE65 cause autosomal recessive childhood-onset severe retinal dystrophy. Nat. Genet. 17: 194-197, 1997. PubMed: 9326941 Debra A Thompson, personal communication |
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| 产品名称： | hE61.f1 |
|---|---|
| 商品货号： | TS190810 |
| Designations： | hE61.f1 |
| GenBank Number： | U18991 |
| Species： | Homo sapiens, human |
| Depositors： | DA Thompson |
| Vector： | Construct size (kb): 5.599999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.599999904632568 Tissue: retinal pigment epithelium Gene product: retinal pigment epithelium-specific protein (65kD) RPE65 |
| Insert Size (kb)： | 2.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 2.7; SmaI--4.4, 1.0; PstI--2.9, 1.8, 0.65. The 2.6kb insert differs from the GenBank entry as follows: A17C, G35C, G36A, G234A, G2573C, T2601C and it contains 24 additional nucleotides at the 3 end. The 24 additional nucleotides (GGG CTG CAG GAA TTC CTG CAG GGG) introduce EcoRI, SmaI, PstI restriction sites at the 3 end of the insert. Inserts detects an insertion/deletion polymorphism at the following nucleotides of the GenBank record G2384del and A2385del. |
| References： | Nicoletti A, et al. Molecular characterization of the human gene encoding abundant 61 kDA protein specific to the retinal pigment epithelium. Hum. Mol. Genet. 4: 641-649, 1995. PubMed: 7633413 Gu SM, et al. Mutations in RPE65 cause autosomal recessive childhood-onset severe retinal dystrophy. Nat. Genet. 17: 194-197, 1997. PubMed: 9326941 Debra A Thompson, personal communication |
