| 产品名称： | Human genomic DNA (HCC38) |
|---|---|
| 商品货号： | TS190898 |
| Product Format： | frozen 10 µg |
| Storage Conditions： | -20°C or colder in a manual defrost freezer. Avoid freeze-thawing. |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Introduction： | Cell Line Description: This cell line was initiated on April 27, 1992 and took 32 months to establish. The cells are negative for expression of Her2-neu but positive for expression of p53. HCC38 was initiated from a 50-year-old white female with a prior history of leiomyosarcoma; her mother died of breast cancer. The tumor was classified as TMN Stage IIB, Grade 3, with 3/28 lymph node metastasis.xa0 HCC38 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 EGP2 and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR). HCC38 contains a homozygous deletion at 3p12. DNA from an EBV- transformed lymphoblastoid cell line, HCC38 BL, from the same patient is available as ATCC CRL-2346D. |
| Quality Control Specifications： | DNA Quality Control Information: ·xa0xa0xa0xa0xa0xa0xa0xa0 > 100 kb in size by agarose gel electrophoresis ·xa0xa0xa0xa0xa0xa0xa0xa0 No RNA detected in the agarose gel ·xa0xa0xa0xa0xa0xa0xa0xa0 A260/A280 ratio ³ 1.8 ·xa0xa0xa0xa0xa0xa0xa0xa0 Tested and verified for PCR amplification and restriction digestion. |
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| 产品名称： | Human genomic DNA (HCC38) |
|---|---|
| 商品货号： | TS190898 |
| Product Format： | frozen 10 µg |
| Storage Conditions： | -20°C or colder in a manual defrost freezer. Avoid freeze-thawing. |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Introduction： | Cell Line Description: This cell line was initiated on April 27, 1992 and took 32 months to establish. The cells are negative for expression of Her2-neu but positive for expression of p53. HCC38 was initiated from a 50-year-old white female with a prior history of leiomyosarcoma; her mother died of breast cancer. The tumor was classified as TMN Stage IIB, Grade 3, with 3/28 lymph node metastasis.xa0 HCC38 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 EGP2 and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR). HCC38 contains a homozygous deletion at 3p12. DNA from an EBV- transformed lymphoblastoid cell line, HCC38 BL, from the same patient is available as ATCC CRL-2346D. |
| Quality Control Specifications： | DNA Quality Control Information: ·xa0xa0xa0xa0xa0xa0xa0xa0 > 100 kb in size by agarose gel electrophoresis ·xa0xa0xa0xa0xa0xa0xa0xa0 No RNA detected in the agarose gel ·xa0xa0xa0xa0xa0xa0xa0xa0 A260/A280 ratio ³ 1.8 ·xa0xa0xa0xa0xa0xa0xa0xa0 Tested and verified for PCR amplification and restriction digestion. |
