| 产品名称： | pSR-1c DJ200 |
|---|---|
| 商品货号： | TS191164 |
| Designations： | pSR-1c DJ200 |
| GenBank Number： | M21410 |
| Species： | Rattus norvegicus, rat |
| Depositors： | Columbia University |
| Insert： | DNA: cDNA Insert lengths(kb): 3.0 Tissue: choroid plexus Gene product: 5-hydroxytryptamine receptor 1c (serotonin receptor) HTR1C |
| Insert Size (kb)： | 3.0 |
| Media： | ATCC Medium 1065 (see below) plus ampicillin (50.0 mcg/ml)
ATCC Medium 1065:
Tryptone (Difco 0123), 10.0 g
Yeast Extract (Difco 0127), 5.0 g
NaCl, 10.0 g
Distilled water, 1.0 L
|
| Related Products： | recombinant clone:ATCC CRL-9651 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): XhoI--7.4, 4.0; EcoRI--8.6, 3.0; HindIII--8.6, 1.6, 1.3; SacI--4.8, 3.8, 1.5, 1.0; SalI--4.7, 3.2, 3.0. The insert contains the following restriction sites (nt position according to the published sequence): HindIII--23, 1616, 1735; SalI--365; SacI--394, 1921. This clone was constructed by subcloning the insert described in the Science paper into the vector pMV7. Contains 700 bp 5 untranslated region, the open reading frame from nt 688 to nt 2068, and part of the 3 untranslated region. Detects a 5.2 kb mRNA in choroid plexus, basal ganglia, hypothalamus, hippocampus, pons medulla and spinal cord. |
| References： | Julius DJ, et al. DNA encoding serotonin 1c (5HT1c) receptor, isolated 5HT1c receptor, mammalian cells expressing same and uses thereof. US Patent 4,985,352 dated Jan 15 1991 Julius D, et al. Molecular characterization of a functional cDNA encoding the serotonin 1c receptor. Science 241: 558-564, 1988. PubMed: 3399891 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pSR-1c DJ200 |
|---|---|
| 商品货号： | TS191164 |
| Designations： | pSR-1c DJ200 |
| GenBank Number： | M21410 |
| Species： | Rattus norvegicus, rat |
| Depositors： | Columbia University |
| Insert： | DNA: cDNA Insert lengths(kb): 3.0 Tissue: choroid plexus Gene product: 5-hydroxytryptamine receptor 1c (serotonin receptor) HTR1C |
| Insert Size (kb)： | 3.0 |
| Media： | ATCC Medium 1065 (see below) plus ampicillin (50.0 mcg/ml)
ATCC Medium 1065:
Tryptone (Difco 0123), 10.0 g
Yeast Extract (Difco 0127), 5.0 g
NaCl, 10.0 g
Distilled water, 1.0 L
|
| Related Products： | recombinant clone:ATCC CRL-9651 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): XhoI--7.4, 4.0; EcoRI--8.6, 3.0; HindIII--8.6, 1.6, 1.3; SacI--4.8, 3.8, 1.5, 1.0; SalI--4.7, 3.2, 3.0. The insert contains the following restriction sites (nt position according to the published sequence): HindIII--23, 1616, 1735; SalI--365; SacI--394, 1921. This clone was constructed by subcloning the insert described in the Science paper into the vector pMV7. Contains 700 bp 5 untranslated region, the open reading frame from nt 688 to nt 2068, and part of the 3 untranslated region. Detects a 5.2 kb mRNA in choroid plexus, basal ganglia, hypothalamus, hippocampus, pons medulla and spinal cord. |
| References： | Julius DJ, et al. DNA encoding serotonin 1c (5HT1c) receptor, isolated 5HT1c receptor, mammalian cells expressing same and uses thereof. US Patent 4,985,352 dated Jan 15 1991 Julius D, et al. Molecular characterization of a functional cDNA encoding the serotonin 1c receptor. Science 241: 558-564, 1988. PubMed: 3399891 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
