| 产品名称： | pNH-Kan/oriT |
|---|---|
| 商品货号： | TS191208 |
| Designations： | pNH-Kan/oriT |
| Species： | Tn5 |
| Depositors： | PN Hengen |
| Vector： | Construct size (kb): 7.199999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 4.5 Gene product: aminoglycoside phosphotransferase(kanamycin resistance, neomycin resistance) aph |
| Insert Size (kb)： | 4.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--4.5, 2.7; BamHI--2.7 (doublet), 1.8; PstI--2.9, 2.7, 1.0, 0.6; EcoRI--4.5, 2.7. Plasmid containing an oriT/kanR cassette bounded by multiple restriction sites for ease in subcloning and for selection in a broad range of gram-negative bacteria. The order of elements in the polylinker is: EcoRI SmaI BamHI XbaI PstI - HindIII insert oriT BamHI SalI PstI kanR/PstI insert HindIII - PstI XbaI BamHI SmaI KpnI EcoRI. |
| References： | Hengen PN, Iyer VN. DNA cassettes containing the origin of transer (oriT) of two broad-host-range transfer systems. BioTechniques 13: 56-62, 1992. PubMed: 1503776 |
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| 产品名称： | pNH-Kan/oriT |
|---|---|
| 商品货号： | TS191208 |
| Designations： | pNH-Kan/oriT |
| Species： | Tn5 |
| Depositors： | PN Hengen |
| Vector： | Construct size (kb): 7.199999809265137 |
| Insert： | DNA: genomic Insert lengths(kb): 4.5 Gene product: aminoglycoside phosphotransferase(kanamycin resistance, neomycin resistance) aph |
| Insert Size (kb)： | 4.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII--4.5, 2.7; BamHI--2.7 (doublet), 1.8; PstI--2.9, 2.7, 1.0, 0.6; EcoRI--4.5, 2.7. Plasmid containing an oriT/kanR cassette bounded by multiple restriction sites for ease in subcloning and for selection in a broad range of gram-negative bacteria. The order of elements in the polylinker is: EcoRI SmaI BamHI XbaI PstI - HindIII insert oriT BamHI SalI PstI kanR/PstI insert HindIII - PstI XbaI BamHI SmaI KpnI EcoRI. |
| References： | Hengen PN, Iyer VN. DNA cassettes containing the origin of transer (oriT) of two broad-host-range transfer systems. BioTechniques 13: 56-62, 1992. PubMed: 1503776 |
