2A3

规格:

货期:

编号:TS191498

品牌:Testobio

产品名称：	2A3
商品货号：	TS191498
Organism：	Homo sapiens, human
Tissue：	pharynx
Product Format：	frozen
Morphology：	epithelial-like
Culture Properties：	adherent
Biosafety Level：	2 Cells contain human papillomavirus (HPV) DNA sequences Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	squamous cell carcinoma
Age：	56 years old
Gender：	male
Ethnicity：	Caucasian
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	(P16) hypodiploid to hypertriploid with modal number = 64
Derivation：	The 2A3 cell line was derived by transfecting ATCC HTB-43 (FaDu) with E6 and E7 genes using PA317 LXSN 16E6/E7. The pLXSN16E6E7 vector contained the human papilloma virus (HPV) type 16 E6 and E7 genes under control of the Moloney murine leukemia virus (MoMuLV) promoter-enhancer sequences. The vector also contained a gene encoding resistance to neomycin transcribed from the SV40 promoter.
Clinical Data：	56 years Caucasian male
Tumorigenic：	Yes
Effects：	Yes, in nude mice; forms well differentiated epidermoid carcinoma (grade I)
Comments：	The 2A3 cell line was derived by transfecting ATCC HTB-43 (FaDu) with E6 and E7 genes using PA317 LXSN 16E6/E7. The pLXSN16E6E7 vector contained the human papilloma virus (HPV) type 16 E6 and E7 genes under control of the Moloney murine leukemia virus (MoMuLV) promoter-enhancer sequences. The vector also contained a gene encoding resistance to neomycin transcribed from the SV40 promoter. This stably transfected head and neck cell line may be used as a basis for animal models for drug development as it was 100% tumorigenic in nude mice while continuing to express E6 oncoprotein in vivo.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 0.2 mg/ml G -418 fetal bovine serum to a final concentration of 10%
Subculturing：	Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca⁺⁺/Mg⁺⁺ free Dulbeccos phosphate-buffered saline (D-PBS) (ATCC 30-2200) or 0.25% Trypsin – 0.53mM EDTA (ATCC 30-2101) solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:3 to 1:6 is recommendedxa0 Medium Renewal: 2 to 3 times per week
Cryopreservation：	Culture medium, 95%; DMSO, 5%
Culture Conditions：	Temperature: 37°C
STR Profile：	Amelogenin: None detected CSF1PO: 12 D13S317: 8, 9 D16S539: 11 D5S818: 12 D7S820: 11, 12 THO1: 8 TPOX: 11 vWA: 15, 17
Isoenzymes：	AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 2 PGM1, 2 PGM3, 1
Population Doubling Time：	51 hours
Name of Depositor：	E Dadachova, Ph.D.
Year of Origin：	June 2010
References：	Harris M, et al. Radioimmunotherapy of experimental head and neck squamous cell carcinoma (HNSCC) with E6-specific antibody using a novel HPV-16 positive HNSCC cell line. Head Neck Oncol. 3(1): 9, 2011. PubMed: 21314983

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2A3

货号: TS191498
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产品名称：	2A3
商品货号：	TS191498
Organism：	Homo sapiens, human
Tissue：	pharynx
Product Format：	frozen
Morphology：	epithelial-like
Culture Properties：	adherent
Biosafety Level：	2 Cells contain human papillomavirus (HPV) DNA sequences Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	squamous cell carcinoma
Age：	56 years old
Gender：	male
Ethnicity：	Caucasian
Storage Conditions：	liquid nitrogen vapor phase
Karyotype：	(P16) hypodiploid to hypertriploid with modal number = 64
Derivation：	The 2A3 cell line was derived by transfecting ATCC HTB-43 (FaDu) with E6 and E7 genes using PA317 LXSN 16E6/E7. The pLXSN16E6E7 vector contained the human papilloma virus (HPV) type 16 E6 and E7 genes under control of the Moloney murine leukemia virus (MoMuLV) promoter-enhancer sequences. The vector also contained a gene encoding resistance to neomycin transcribed from the SV40 promoter.
Clinical Data：	56 years Caucasian male
Tumorigenic：	Yes
Effects：	Yes, in nude mice; forms well differentiated epidermoid carcinoma (grade I)
Comments：	The 2A3 cell line was derived by transfecting ATCC HTB-43 (FaDu) with E6 and E7 genes using PA317 LXSN 16E6/E7. The pLXSN16E6E7 vector contained the human papilloma virus (HPV) type 16 E6 and E7 genes under control of the Moloney murine leukemia virus (MoMuLV) promoter-enhancer sequences. The vector also contained a gene encoding resistance to neomycin transcribed from the SV40 promoter. This stably transfected head and neck cell line may be used as a basis for animal models for drug development as it was 100% tumorigenic in nude mice while continuing to express E6 oncoprotein in vivo.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 0.2 mg/ml G -418 fetal bovine serum to a final concentration of 10%
Subculturing：	Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca⁺⁺/Mg⁺⁺ free Dulbeccos phosphate-buffered saline (D-PBS) (ATCC 30-2200) or 0.25% Trypsin – 0.53mM EDTA (ATCC 30-2101) solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: 1:3 to 1:6 is recommendedxa0 Medium Renewal: 2 to 3 times per week
Cryopreservation：	Culture medium, 95%; DMSO, 5%
Culture Conditions：	Temperature: 37°C
STR Profile：	Amelogenin: None detected CSF1PO: 12 D13S317: 8, 9 D16S539: 11 D5S818: 12 D7S820: 11, 12 THO1: 8 TPOX: 11 vWA: 15, 17
Isoenzymes：	AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 2 PGM1, 2 PGM3, 1
Population Doubling Time：	51 hours
Name of Depositor：	E Dadachova, Ph.D.
Year of Origin：	June 2010
References：	Harris M, et al. Radioimmunotherapy of experimental head and neck squamous cell carcinoma (HNSCC) with E6-specific antibody using a novel HPV-16 positive HNSCC cell line. Head Neck Oncol. 3(1): 9, 2011. PubMed: 21314983