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HHCPP06
HHCPP06
规格:
货期:
编号:TS191520
品牌:Testobio
产品名称: HHCPP06
商品货号: TS191520
Designations: HHCPP06
GenBank Number:

M78186

Species: Homo sapiens, human
Vector:
Construct size (kb): 3.960000038146973
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D0S1182E
Genomic copy number: unique
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Sequence homology: Homo sapiens postsynaptic density protein
Homo sapiens discs-large tumor suppressor
Tissue: hippocampus, female, 2 years, oligo-dT and random primed
(Stratagene 936205), subtracted
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.0
Cross references: DNA Seq. Acc.: M78186; EST: EST01779; Primer: M13 Forward;
THC id: THC106852
DNA Seq. Acc.: M96853
Nucleotides 1-342 of the insert correspond to
nucleotides 1748-1407 of M96853 with 92% identity.
Insert lengths(kb): 1.0
Tissue: hippocampus, female, 2 years, oligo-dT and random primed (Stratagene 936205), subtracted
Gene product: discs-large tumor suppressor PSD95*
Insert Size (kb): 1.0
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): HindIII--4.0; EcoRI--2.9, 1.1.
Based on the alignment with the sequence of the rat cDNA, GenBank accession M96853 (3083 nt, CDS 52-2232), this clone appears to contain coding sequence for the amino terminal portion of the protein.
EST01779 matches GenPept accession DRODLGA_1 with 62.96% identity and 87.03% similarity over the sequence range: 164-3.
This sequence record is for Drosophila melanogaster discs-large tumor suppressor.
References:

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

J C Venter, personal communication

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HHCPP06

  • 货号: TS191520
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: HHCPP06
商品货号: TS191520
Designations: HHCPP06
GenBank Number:

M78186

Species: Homo sapiens, human
Vector:
Construct size (kb): 3.960000038146973
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D0S1182E
Genomic copy number: unique
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Sequence homology: Homo sapiens postsynaptic density protein
Homo sapiens discs-large tumor suppressor
Tissue: hippocampus, female, 2 years, oligo-dT and random primed
(Stratagene 936205), subtracted
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.0
Cross references: DNA Seq. Acc.: M78186; EST: EST01779; Primer: M13 Forward;
THC id: THC106852
DNA Seq. Acc.: M96853
Nucleotides 1-342 of the insert correspond to
nucleotides 1748-1407 of M96853 with 92% identity.
Insert lengths(kb): 1.0
Tissue: hippocampus, female, 2 years, oligo-dT and random primed (Stratagene 936205), subtracted
Gene product: discs-large tumor suppressor PSD95*
Insert Size (kb): 1.0
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): HindIII--4.0; EcoRI--2.9, 1.1.
Based on the alignment with the sequence of the rat cDNA, GenBank accession M96853 (3083 nt, CDS 52-2232), this clone appears to contain coding sequence for the amino terminal portion of the protein.
EST01779 matches GenPept accession DRODLGA_1 with 62.96% identity and 87.03% similarity over the sequence range: 164-3.
This sequence record is for Drosophila melanogaster discs-large tumor suppressor.
References:

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

J C Venter, personal communication

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