Escherichia coli
Escherichia coli
规格:
货期:
编号:TS192413
品牌:Testobio
| 产品名称： | Escherichia coli 1046 (pCRE1) |
|---|---|
| 商品货号： | TS192413 |
| Designations： | Escherichia coli 1046 (pCRE1) |
| Depositors： | IN Maruyama |
| Applications： | Produces protein recombinase, site-specific |
| Vector： | Construct size (kb): 5.960
Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: ScaI Vector end: ScaI Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMIxa0BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaIxa0PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | Genome: bacteriophage P1
Gene symbol: cre
Gene name: recombinase, site-specific Contains complete coding sequence?: U Type of DNA: genomic Insert end: PvuII Insert end: PvuII Insert size (kb): 1.6 Cross references: Gene product: recombinase, site-specific cre
Target Gene: recombinase, site-specific |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Related Products： | Component of: ATCC 77369 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Freeze-dried E. coli 1046 |
| Comments： | This E. coli contains the pCRE1 plasmid and expresses the Cre recombinase from a lacZ promoter.xa0 Insert-containing lambdaMGU2 (77367) cannot grow productively in this RecA- strain, so excised phagemid are recovered as ampR surviviors of phage infection. Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 2.05; XmnI--3.1, 2.4, 0.45; HindIII--5.2, 0.75; BamHI--4.4, 1.55; EcoRI/PstI--3.5, 1.4, 0.7, 0.35. |
| References： | Maruyama IN, Brenner S. A selective lambda phage cloning vector with automatic excision of the insert in a plasmid. Gene 120: 135-141, 1992. PubMed: 1327972 |
首页 >
产品中心
>
微生物培养
>
菌株
>
null
>
Escherichia coli
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | Escherichia coli 1046 (pCRE1) |
|---|---|
| 商品货号： | TS192413 |
| Designations： | Escherichia coli 1046 (pCRE1) |
| Depositors： | IN Maruyama |
| Applications： | Produces protein recombinase, site-specific |
| Vector： | Construct size (kb): 5.960
Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: ScaI Vector end: ScaI Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMIxa0BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaIxa0PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | Genome: bacteriophage P1
Gene symbol: cre
Gene name: recombinase, site-specific Contains complete coding sequence?: U Type of DNA: genomic Insert end: PvuII Insert end: PvuII Insert size (kb): 1.6 Cross references: Gene product: recombinase, site-specific cre
Target Gene: recombinase, site-specific |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Related Products： | Component of: ATCC 77369 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Freeze-dried E. coli 1046 |
| Comments： | This E. coli contains the pCRE1 plasmid and expresses the Cre recombinase from a lacZ promoter.xa0 Insert-containing lambdaMGU2 (77367) cannot grow productively in this RecA- strain, so excised phagemid are recovered as ampR surviviors of phage infection. Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 2.05; XmnI--3.1, 2.4, 0.45; HindIII--5.2, 0.75; BamHI--4.4, 1.55; EcoRI/PstI--3.5, 1.4, 0.7, 0.35. |
| References： | Maruyama IN, Brenner S. A selective lambda phage cloning vector with automatic excision of the insert in a plasmid. Gene 120: 135-141, 1992. PubMed: 1327972 |
