| 产品名称： | FB8H3 Mab8H3 |
|---|---|
| 商品货号： | TS192573 |
| Organism： | Mus musculus (B cell); Mus musculus (lymphoma), mouse (B cell); mouse (lymphoma) |
| Tissue： | spleen |
| Cell Type： | hybridoma: B lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | BALB/c |
| Applications： | The FB8H3 hybridoma was produced by fusing P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice that had been immunized with a fumonisinB1 - BSA conjugate. The antibody is specific for fumonisin mycotoxins, and is useful for detecting this class of mycotoxins in maize and maize products. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The FB8H3 hybridoma was produced by fusing P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice that had been immunized with a fumonisinB1 - BSA conjugate. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against fumonisin B1 mycotoxin. |
| Cellular Products： | immunoglobulin; monoclonal antibody; against fumonisin B1 mycotoxin. |
| Comments： | The FB8H3 hybridoma was produced by fusing P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice that had been immunized with a fumonisinB1 - BSA conjugate. The antibody is specific for fumonisin mycotoxins, and is useful for detecting this class of mycotoxins in maize and maize products. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2xa0to 3 x 105 viable cells/mL.xa0 Maintain cultures at a cell concentration between 2 x 105 and 1 x 106 cells/mL.xa0 Do not allow the cell concentration to exceed 1 x 106 cells/mL. Medium Renewal:xa0Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | mouse IgG1 |
| Name of Depositor： | RA Shelby |
| Deposited As： | mouse (B cell); mouse (lymphoma) |
| References： | Shelby RA, et al. Comparison of thin-layer chromatography and competitive immunoassay methods for detecting fumonisin on maize. J. Agric. Food Chem. 42: 2064-2067, 1994. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |
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| 产品名称： | FB8H3 Mab8H3 |
|---|---|
| 商品货号： | TS192573 |
| Organism： | Mus musculus (B cell); Mus musculus (lymphoma), mouse (B cell); mouse (lymphoma) |
| Tissue： | spleen |
| Cell Type： | hybridoma: B lymphoblast |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | BALB/c |
| Applications： | The FB8H3 hybridoma was produced by fusing P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice that had been immunized with a fumonisinB1 - BSA conjugate. The antibody is specific for fumonisin mycotoxins, and is useful for detecting this class of mycotoxins in maize and maize products. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The FB8H3 hybridoma was produced by fusing P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice that had been immunized with a fumonisinB1 - BSA conjugate. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against fumonisin B1 mycotoxin. |
| Cellular Products： | immunoglobulin; monoclonal antibody; against fumonisin B1 mycotoxin. |
| Comments： | The FB8H3 hybridoma was produced by fusing P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice that had been immunized with a fumonisinB1 - BSA conjugate. The antibody is specific for fumonisin mycotoxins, and is useful for detecting this class of mycotoxins in maize and maize products. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2xa0to 3 x 105 viable cells/mL.xa0 Maintain cultures at a cell concentration between 2 x 105 and 1 x 106 cells/mL.xa0 Do not allow the cell concentration to exceed 1 x 106 cells/mL. Medium Renewal:xa0Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | mouse IgG1 |
| Name of Depositor： | RA Shelby |
| Deposited As： | mouse (B cell); mouse (lymphoma) |
| References： | Shelby RA, et al. Comparison of thin-layer chromatography and competitive immunoassay methods for detecting fumonisin on maize. J. Agric. Food Chem. 42: 2064-2067, 1994. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |
