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HFBCC12
HFBCC12
规格:
货期:
编号:TS192733
品牌:Testobio
产品名称: HFBCC12
商品货号: TS192733
Designations: HFBCC12
GenBank Number:

M55409

M77902

Species: Homo sapiens, human
Vector:
Construct size (kb): 4.099999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene name: translation elongation factor 1, gamma
Contains complete coding sequence?: U
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.1
Cross references: DNA Seq. Acc.: M55409; EST: EST01486
Nucleotides 1-423 of the insert correspond to
nucleotides 131-553 of M55409 with 99% identity.
DNA Seq. Acc.: M77902; EST: EST01486; Primer: M13 Forward;
THC id: THC124603
Insert lengths(kb): 1.100000023841858
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Gene product: translation elongation factor 1, gamma
Insert Size (kb): 1.100
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.1; PstI--4.1; HindIII--4.1.
Based on the alignment with GenBank accession M55409 (1155 nt, CDS <1-1079), EST01486 appears to contain part of the coding region.
References:

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

J C Venter, personal communication

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HFBCC12

  • 货号: TS192733
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  • 品牌 : TESTOBIO
产品名称: HFBCC12
商品货号: TS192733
Designations: HFBCC12
GenBank Number:

M55409

M77902

Species: Homo sapiens, human
Vector:
Construct size (kb): 4.099999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene name: translation elongation factor 1, gamma
Contains complete coding sequence?: U
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.1
Cross references: DNA Seq. Acc.: M55409; EST: EST01486
Nucleotides 1-423 of the insert correspond to
nucleotides 131-553 of M55409 with 99% identity.
DNA Seq. Acc.: M77902; EST: EST01486; Primer: M13 Forward;
THC id: THC124603
Insert lengths(kb): 1.100000023841858
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Gene product: translation elongation factor 1, gamma
Insert Size (kb): 1.100
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.1; PstI--4.1; HindIII--4.1.
Based on the alignment with GenBank accession M55409 (1155 nt, CDS <1-1079), EST01486 appears to contain part of the coding region.
References:

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

J C Venter, personal communication

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