| 产品名称： | pHPS9 |
|---|---|
| 商品货号： | TS192860 |
| Designations： | pHPS9 |
| Depositors： | S Bron |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.6999998092651370 Vector: pHPS9 (plasmid) Promoters: Promoter P59 Construction: pGKH1, pHP13-2 Marker(s):cmlR,eryR Construct size (kb): 5.699999809265137 Features: insert detection: lacZ marker(s): cmlR, eryR promoter: P59 replicon: pMB1, pTA1060 |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.7, EcoRI--5.7, PstI--4.9, 0.7. Cloning into the NdeI, NheI, BamHI, SmaI or EcoRI sites inactivates lacZalpha. The NcoI site interrupts the chloramphenicol resistance sequence. This is the preferred strain for isolating the plasmid, because the copy number is higher in E. coli than in B. subtilis. Permits alpha-complementation to identify recombinants when used with Bacillus subtilis 6GM15, and alpha-complementation with plasmid marker rescue when used with Bacillis subtilis 6GM15pHPS9R (ATCC 37818). The cat86::lacZalpha fusion is in-frame. Expression is controlled by the P59 promoter from Lactococcus lactis subsp. cremoris Wg2. |
| Media： | ATCC® Medium 2938: LB Agar/Broth, Miller w/200ug/ml Erythromycin |
| Growth Conditions： | Temperature: 37°C
Atmosphere: Aerobic |
| References： | Haima P, et al. Development of a beta-galactosidase alpha-complementation system for molecular cloning in Bacillus subtilis. Gene 86: 63-69, 1990. PubMed: 2107125 Haima P, et al. An improved beta-galactosidase alpha-complementation system for molecular cloning in Bacillus subtilis. Gene 93: 41-47, 1990. PubMed: 2121609 Haima P, et al. Novel plasmid marker rescue transformation system for molecular cloning in Bacillus subtilis enabling direct selection of recombinants. Mol. Gen. Genet. 223: 185-191, 1990. PubMed: 2123518 Sierd Bron, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |
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| 产品名称： | pHPS9 |
|---|---|
| 商品货号： | TS192860 |
| Designations： | pHPS9 |
| Depositors： | S Bron |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 5.6999998092651370 Vector: pHPS9 (plasmid) Promoters: Promoter P59 Construction: pGKH1, pHP13-2 Marker(s):cmlR,eryR Construct size (kb): 5.699999809265137 Features: insert detection: lacZ marker(s): cmlR, eryR promoter: P59 replicon: pMB1, pTA1060 |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.7, EcoRI--5.7, PstI--4.9, 0.7. Cloning into the NdeI, NheI, BamHI, SmaI or EcoRI sites inactivates lacZalpha. The NcoI site interrupts the chloramphenicol resistance sequence. This is the preferred strain for isolating the plasmid, because the copy number is higher in E. coli than in B. subtilis. Permits alpha-complementation to identify recombinants when used with Bacillus subtilis 6GM15, and alpha-complementation with plasmid marker rescue when used with Bacillis subtilis 6GM15pHPS9R (ATCC 37818). The cat86::lacZalpha fusion is in-frame. Expression is controlled by the P59 promoter from Lactococcus lactis subsp. cremoris Wg2. |
| Media： | ATCC® Medium 2938: LB Agar/Broth, Miller w/200ug/ml Erythromycin |
| Growth Conditions： | Temperature: 37°C
Atmosphere: Aerobic |
| References： | Haima P, et al. Development of a beta-galactosidase alpha-complementation system for molecular cloning in Bacillus subtilis. Gene 86: 63-69, 1990. PubMed: 2107125 Haima P, et al. An improved beta-galactosidase alpha-complementation system for molecular cloning in Bacillus subtilis. Gene 93: 41-47, 1990. PubMed: 2121609 Haima P, et al. Novel plasmid marker rescue transformation system for molecular cloning in Bacillus subtilis enabling direct selection of recombinants. Mol. Gen. Genet. 223: 185-191, 1990. PubMed: 2123518 Sierd Bron, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |
