| 产品名称： | 3T3-L1 MBX |
|---|---|
| 商品货号： | TS193303 |
| Organism： | Mus musculus, mouse |
| Tissue： | embryo |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | adipogenesis and adipocyte differentiation, xa0diabetes and obesity research areas |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | 3T3 L1-MBX clone was derived from 3T3-L1 (CL-173) to ensure close to 100% differentiation to adipocytes and great response to insulin |
| Comments： | The 3T3 L1-MBX clone was derived from 3T3-L1 (ATCC CL-173) to ensure close to 100% differentiation to adipocytes and great response to insulin. In addition, 3T3 L1-MBX has a great insulin-stimulated glucose uptake response which is about 8-10 fold window with sub-maximal insulin concentration in 2-deoxyglucose uptake assay (2-DOG). This cell line would be a valuable tool for researchers who are interested in diabetes and obesity research areas. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Note: Never allow culture to become completely confluent. Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | 90% FBS supplemented with 10% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Name of Depositor： | CymaBay Therapeutics (formerly Metabolex, Inc.) & Choi Y |
| References： | Gregoire FM, et al. MBX-102/JNJ39659100, a novel peroxisome proliferator-activated receptor-ligand with weak transactivation activity retains antidiabetic properties in the absence of weight gain and edema. Mol. Endocrinol. 23(7): 975-88, 2009. PubMed: 19389808 |
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | 3T3-L1 MBX |
|---|---|
| 商品货号： | TS193303 |
| Organism： | Mus musculus, mouse |
| Tissue： | embryo |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | adipogenesis and adipocyte differentiation, xa0diabetes and obesity research areas |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | 3T3 L1-MBX clone was derived from 3T3-L1 (CL-173) to ensure close to 100% differentiation to adipocytes and great response to insulin |
| Comments： | The 3T3 L1-MBX clone was derived from 3T3-L1 (ATCC CL-173) to ensure close to 100% differentiation to adipocytes and great response to insulin. In addition, 3T3 L1-MBX has a great insulin-stimulated glucose uptake response which is about 8-10 fold window with sub-maximal insulin concentration in 2-deoxyglucose uptake assay (2-DOG). This cell line would be a valuable tool for researchers who are interested in diabetes and obesity research areas. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Note: Never allow culture to become completely confluent. Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | 90% FBS supplemented with 10% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Name of Depositor： | CymaBay Therapeutics (formerly Metabolex, Inc.) & Choi Y |
| References： | Gregoire FM, et al. MBX-102/JNJ39659100, a novel peroxisome proliferator-activated receptor-ligand with weak transactivation activity retains antidiabetic properties in the absence of weight gain and edema. Mol. Endocrinol. 23(7): 975-88, 2009. PubMed: 19389808 |
