| 产品名称： | pMDIII |
|---|---|
| 商品货号： | TS193405 |
| Designations： | pMDIII |
| Depositors： | H Wolf |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.0000000000000000 Vector: pMDIII (plasmid) Promoters: Promoter SV40 early Marker(s):ampR Construct size (kb): 7.0 Features: marker(s): ampR promoter: SV40 early replicon: pMB1 enhancer: SV40 |
| Applications： | expression vector shuttle vector |
| Comments： | A vector for expression of cloned sequences under the control of the SV40 early promoter in mammalian cell culture selected for dhfr+ phenotype. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Breathnach R, Harris BA. Plasmids for the cloning and expression of full-length double-stranded cDNAs under the control of the SV40 early or late gene promoter. Nucleic Acids Res. 11: 7119-7136, 1983. PubMed: 6314276 Kaufman RJ, Sharp PA. Construction of a modular dihydrofolate reductase cDNA gene: analysis of signals utilized for efficient expression. Mol. Cell. Biol. 2: 1304-1319, 1982. PubMed: 6131378 Motz M, et al. Expression of the Epstein-Barr virus major membrane protein in Chinese hamster ovary cells. Gene 44: 353-359, 1986. PubMed: 3023198 |
| Shipped： | freeze-dried |
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| 产品名称： | pMDIII |
|---|---|
| 商品货号： | TS193405 |
| Designations： | pMDIII |
| Depositors： | H Wolf |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.0000000000000000 Vector: pMDIII (plasmid) Promoters: Promoter SV40 early Marker(s):ampR Construct size (kb): 7.0 Features: marker(s): ampR promoter: SV40 early replicon: pMB1 enhancer: SV40 |
| Applications： | expression vector shuttle vector |
| Comments： | A vector for expression of cloned sequences under the control of the SV40 early promoter in mammalian cell culture selected for dhfr+ phenotype. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Breathnach R, Harris BA. Plasmids for the cloning and expression of full-length double-stranded cDNAs under the control of the SV40 early or late gene promoter. Nucleic Acids Res. 11: 7119-7136, 1983. PubMed: 6314276 Kaufman RJ, Sharp PA. Construction of a modular dihydrofolate reductase cDNA gene: analysis of signals utilized for efficient expression. Mol. Cell. Biol. 2: 1304-1319, 1982. PubMed: 6131378 Motz M, et al. Expression of the Epstein-Barr virus major membrane protein in Chinese hamster ovary cells. Gene 44: 353-359, 1986. PubMed: 3023198 |
| Shipped： | freeze-dried |
