| 产品名称： | pBH302 |
|---|---|
| 商品货号： | TS193652 |
| Designations： | pBH302 |
| Species： | Homo sapiens, human |
| Depositors： | WE Bradley |
| Vector： | Construct size (kb): 5.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.0 Tissue: placenta Gene product: thyroid hormone receptor, beta (avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 2) THRB Alleles: A2, A2, A1, B1, B2, D1, E1, E2, A1, B1, B2, D1, E1, E2, D2, D2 |
| Insert Size (kb)： | 2.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 2.0; EcoRI--4.9; HindIII--4.9; PstI--4.1, 0.8; EcoRI/PstI--2.9, 1.2, 0.8. The insert was subcloned from lambdaPGE302 and includes a short exon in the 5 untranslated region of erbAbeta. This probe contains some low repeat sequences. Good results are obtained by hybridizing in the presence of 25 ug/ml human DNA, although it may not be necessary. Enzyme(s) not detecting polymorphism: EcoRI, MspI, PstI, TaqI, PvuII, BamHI. Enzyme(s) not detecting polymorphism: EcoRI, TaqI, PvuII, BamHI, BglII, RsaI, HinfI. |
| References： | Gareau JL, et al. A frequent HindIII RFLP on chromosome 3p21-25 detected by a genomic erbAbeta sequence. Nucleic Acids Res. 16: 1223, 1988. PubMed: 2894009 Usala SJ, et al. Tight linkage between the syndrome of generalized thyroid hormone resistance and the human c-erbA(beta) gene. Mol. Endocrinol. 2: 1217-1220, 1988. PubMed: 2905763 Botto M, et al. EcoRI polymorphism in the human third complement component (C3) gene. Nucleic Acids Res. 18: 2833, 1990. PubMed: 1971096 Hosoe S, et al. Localization of the Von Hippel-Lindau disease gene to a small region of chromosome 3. Genomics 8: 634-640, 1990. PubMed: 2276737 Douglas JB, et al. A pulsed-field gel electrophoresis map locates the polymorphic probes for ERBA2 and ErbAbeta within 120 kb of each other, confirming that THRB (formerly ERBA2) maps to chromosome 3. Genomics 9: 398-400, 1991. PubMed: 1672299 |
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| 产品名称： | pBH302 |
|---|---|
| 商品货号： | TS193652 |
| Designations： | pBH302 |
| Species： | Homo sapiens, human |
| Depositors： | WE Bradley |
| Vector： | Construct size (kb): 5.0 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.0 Tissue: placenta Gene product: thyroid hormone receptor, beta (avian erythroblastic leukemia viral (v-erb-a) oncogene homolog 2) THRB Alleles: A2, A2, A1, B1, B2, D1, E1, E2, A1, B1, B2, D1, E1, E2, D2, D2 |
| Insert Size (kb)： | 2.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 2.0; EcoRI--4.9; HindIII--4.9; PstI--4.1, 0.8; EcoRI/PstI--2.9, 1.2, 0.8. The insert was subcloned from lambdaPGE302 and includes a short exon in the 5 untranslated region of erbAbeta. This probe contains some low repeat sequences. Good results are obtained by hybridizing in the presence of 25 ug/ml human DNA, although it may not be necessary. Enzyme(s) not detecting polymorphism: EcoRI, MspI, PstI, TaqI, PvuII, BamHI. Enzyme(s) not detecting polymorphism: EcoRI, TaqI, PvuII, BamHI, BglII, RsaI, HinfI. |
| References： | Gareau JL, et al. A frequent HindIII RFLP on chromosome 3p21-25 detected by a genomic erbAbeta sequence. Nucleic Acids Res. 16: 1223, 1988. PubMed: 2894009 Usala SJ, et al. Tight linkage between the syndrome of generalized thyroid hormone resistance and the human c-erbA(beta) gene. Mol. Endocrinol. 2: 1217-1220, 1988. PubMed: 2905763 Botto M, et al. EcoRI polymorphism in the human third complement component (C3) gene. Nucleic Acids Res. 18: 2833, 1990. PubMed: 1971096 Hosoe S, et al. Localization of the Von Hippel-Lindau disease gene to a small region of chromosome 3. Genomics 8: 634-640, 1990. PubMed: 2276737 Douglas JB, et al. A pulsed-field gel electrophoresis map locates the polymorphic probes for ERBA2 and ErbAbeta within 120 kb of each other, confirming that THRB (formerly ERBA2) maps to chromosome 3. Genomics 9: 398-400, 1991. PubMed: 1672299 |
