| 产品名称： | pDRD2E5.6 |
|---|---|
| 商品货号： | TS193684 |
| Designations： | pDRD2E5.6 |
| Species： | Homo sapiens, human |
| Depositors： | JR Kidd |
| Vector： | Construct size (kb): 8.5600004196167 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript II SK Intact vector size: 2.961 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR promoter: lac, T7, T3 replicon: pMB1, f1 Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: DRD2 Genomic copy number: unique Gene name: dopamine receptor D2 Contains complete coding sequence?: U Chromosome: 11; Localization: 11 q22.2-q22.3 Type of DNA: genomic Insert 5 end: EcoRI Insert 3 end: EcoRI Insert size (kb): 5.6 Cross references: Insert lengths(kb): 5.599999904632568 Gene product: dopamine receptor D2 DRD2 Alleles: A1, B1, B2, A2 |
| Insert Size (kb)： | 5.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.8, 3.0; BamHI--8.0, 0.6; HindIII--8.6; PstI--4.0, 1.5, 1.35, 0.8, 0.5, 0.3; HindIII/PstI--3.4, 1.5, 1.3, 0.8, 0.8, 0.5, 0.3. The insert contains a 4.5Kb TaqI fragment. The downstream portion of pDRD2E5.6 overlaps the Suarez clone phD2-254. |
| References： | Hauge XY, et al. Detection and characterization of additional DNA polymorphisms in the dopamine D2 receptor gene. Genomics 10: 527-530, 1991. PubMed: 1679742 Suarez BK, et al. Linkage Disequilibria at the D2 dopamine receptor (DRD2) in alcoholics and controls. Genomics 19: 12-20, 1994. PubMed: 7910578 Judith R Kidd, personal communication |
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| 产品名称： | pDRD2E5.6 |
|---|---|
| 商品货号： | TS193684 |
| Designations： | pDRD2E5.6 |
| Species： | Homo sapiens, human |
| Depositors： | JR Kidd |
| Vector： | Construct size (kb): 8.5600004196167 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript II SK Intact vector size: 2.961 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ marker(s): ampR promoter: lac, T7, T3 replicon: pMB1, f1 Cross references: |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: DRD2 Genomic copy number: unique Gene name: dopamine receptor D2 Contains complete coding sequence?: U Chromosome: 11; Localization: 11 q22.2-q22.3 Type of DNA: genomic Insert 5 end: EcoRI Insert 3 end: EcoRI Insert size (kb): 5.6 Cross references: Insert lengths(kb): 5.599999904632568 Gene product: dopamine receptor D2 DRD2 Alleles: A1, B1, B2, A2 |
| Insert Size (kb)： | 5.600 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--5.8, 3.0; BamHI--8.0, 0.6; HindIII--8.6; PstI--4.0, 1.5, 1.35, 0.8, 0.5, 0.3; HindIII/PstI--3.4, 1.5, 1.3, 0.8, 0.8, 0.5, 0.3. The insert contains a 4.5Kb TaqI fragment. The downstream portion of pDRD2E5.6 overlaps the Suarez clone phD2-254. |
| References： | Hauge XY, et al. Detection and characterization of additional DNA polymorphisms in the dopamine D2 receptor gene. Genomics 10: 527-530, 1991. PubMed: 1679742 Suarez BK, et al. Linkage Disequilibria at the D2 dopamine receptor (DRD2) in alcoholics and controls. Genomics 19: 12-20, 1994. PubMed: 7910578 Judith R Kidd, personal communication |
