| 产品名称： | c1-26 cosmid 1-26 |
|---|---|
| 商品货号： | TS193701 |
| Designations： | c1-26 cosmid 1-26 |
| Species： | Homo sapiens, human |
| Depositors： | M Litt |
| Applications： | Probe used was a 7.6 kb TaqI fragment. |
| Vector： | Construct size (kb): 51.0 |
| Insert： | DNA: genomic Insert lengths(kb): 40.0 Gene product: DNA Segment, single copy D17S20 Alleles: B1, B2, A1, C1, C3, D1, D2, C2, A2 |
| Insert Size (kb)： | 40.0 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--19.4, 8.8 doublet, 6.5, 2.7, 0.8; EcoRI--15, 8.8, 7.5, 4.3, 3.4, 2.5, 2.1, 1.9, 1.4, 1.2, 1.1, 1, 0.8; SalI--greater than 25; BglI--11, 7.5, 3.8, 2.1, 1.7, 1.3, 0.8. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Probe used was a 7.6 kb TaqI fragment. Probe must be prehybridized with excess human DNA to compete out repeated sequences. Loci A, C, and D are in strong linkage disequilibrium. 29/40 (72%) individuals show heterozygosity at 1 or more loci. EcoRI detects an insertion-deletion polymorphism also detected by BamHI and BstEII. |
| References： | Litt M, White RL. A highly polymorphic locus in human DNA revealed by cosmid-derived probes. Proc. Natl. Acad. Sci. USA 82: 6206-6210, 1985. PubMed: 2994065 Buroker NE, et al. Cosmid 1-26 defines four RFLPs on chromosome 17q23-qter HGM9 No. D17S20. Nucleic Acids Res. 15: 9097, 1987. PubMed: 2891105 |
产品中心 Product Center
联系我们CONTACT US
-
0574-87917803
24小时服务热线 -
testobio@163.com
销售邮箱 -
浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
地址
联系方式
-
章丽敏
17757489010 -
朱静
18067106081 -
李晴
18067408036 -
李晶晶
13375187189 -
周雪
13372109301
| 产品名称： | c1-26 cosmid 1-26 |
|---|---|
| 商品货号： | TS193701 |
| Designations： | c1-26 cosmid 1-26 |
| Species： | Homo sapiens, human |
| Depositors： | M Litt |
| Applications： | Probe used was a 7.6 kb TaqI fragment. |
| Vector： | Construct size (kb): 51.0 |
| Insert： | DNA: genomic Insert lengths(kb): 40.0 Gene product: DNA Segment, single copy D17S20 Alleles: B1, B2, A1, C1, C3, D1, D2, C2, A2 |
| Insert Size (kb)： | 40.0 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--19.4, 8.8 doublet, 6.5, 2.7, 0.8; EcoRI--15, 8.8, 7.5, 4.3, 3.4, 2.5, 2.1, 1.9, 1.4, 1.2, 1.1, 1, 0.8; SalI--greater than 25; BglI--11, 7.5, 3.8, 2.1, 1.7, 1.3, 0.8. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Probe used was a 7.6 kb TaqI fragment. Probe must be prehybridized with excess human DNA to compete out repeated sequences. Loci A, C, and D are in strong linkage disequilibrium. 29/40 (72%) individuals show heterozygosity at 1 or more loci. EcoRI detects an insertion-deletion polymorphism also detected by BamHI and BstEII. |
| References： | Litt M, White RL. A highly polymorphic locus in human DNA revealed by cosmid-derived probes. Proc. Natl. Acad. Sci. USA 82: 6206-6210, 1985. PubMed: 2994065 Buroker NE, et al. Cosmid 1-26 defines four RFLPs on chromosome 17q23-qter HGM9 No. D17S20. Nucleic Acids Res. 15: 9097, 1987. PubMed: 2891105 |
