| 产品名称： | Human echovirus 12 |
|---|---|
| 商品货号： | TS193887 |
| Classification： | Picornaviridae, Enterovirus, Human enterovirus B |
| Deposited As： | Echovirus 12 |
| Agent： | Human echovirus 12 |
| Strain： | Travis 2-85 |
| Common Name： | Human echovirus 12 |
| Positive for Mycoplasma： | No |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | derived from existing strain |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder (or colder) |
| Effect on Host： | Yes, in vitro effects: Cytopathic effects, refractile rounding and sloughing of cells CPE, refractile rounding and sloughing of cells Yes, in vitro effects: agglutinates human type O red blood cells |
| Growth Medium： | Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
|
| Recommended Host： | Recommended Host:xa0 LLC-MK2 (ATCC CCL-7.1) |
| Growth Conditions： | Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C Duration: 2-5 days; For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent). |
| Cryopreservation： | Storage temperature: -70°C |
| Name of Depositor： | ATCC, HA Wenner, PS Kamitsuka |
| Special Collection： | ATCC |
| Source： | derived from existing strain |
| References： | Hammon WM, et al. Problems raised by certain ECHO viruses in the attempted laboratory detection of poliomyelitis virus infection. Ann. N.Y. Acad. Sci. 67: 304-310, 1957. PubMed: 13411968 or colder VR-1563 was created by solvent treating Mycoplasma orale positive VR-42 which was isolated in 1952 from a rectal swab taken from a 6-year-old healthy American male, Philippine Islands and passaging it three times and finding no detectable Mycoplasma by PCR. This holding has no detectable mycoplasma by PCR. or colder |
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| 产品名称： | Human echovirus 12 |
|---|---|
| 商品货号： | TS193887 |
| Classification： | Picornaviridae, Enterovirus, Human enterovirus B |
| Deposited As： | Echovirus 12 |
| Agent： | Human echovirus 12 |
| Strain： | Travis 2-85 |
| Common Name： | Human echovirus 12 |
| Positive for Mycoplasma： | No |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | derived from existing strain |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder (or colder) |
| Effect on Host： | Yes, in vitro effects: Cytopathic effects, refractile rounding and sloughing of cells CPE, refractile rounding and sloughing of cells Yes, in vitro effects: agglutinates human type O red blood cells |
| Growth Medium： | Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
|
| Recommended Host： | Recommended Host:xa0 LLC-MK2 (ATCC CCL-7.1) |
| Growth Conditions： | Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C Duration: 2-5 days; For best results cells should be 24 to 48 hours old and 80-90% confluent (not 100% confluent). |
| Cryopreservation： | Storage temperature: -70°C |
| Name of Depositor： | ATCC, HA Wenner, PS Kamitsuka |
| Special Collection： | ATCC |
| Source： | derived from existing strain |
| References： | Hammon WM, et al. Problems raised by certain ECHO viruses in the attempted laboratory detection of poliomyelitis virus infection. Ann. N.Y. Acad. Sci. 67: 304-310, 1957. PubMed: 13411968 or colder VR-1563 was created by solvent treating Mycoplasma orale positive VR-42 which was isolated in 1952 from a rectal swab taken from a 6-year-old healthy American male, Philippine Islands and passaging it three times and finding no detectable Mycoplasma by PCR. This holding has no detectable mycoplasma by PCR. or colder |
