| 产品名称： | pv-sis SISR12 |
|---|---|
| 商品货号： | TS194271 |
| Designations： | pv-sis SISR12 |
| GenBank Number： | J02396 |
| Species： | simian sarcoma virus (type C) |
| Depositors： | HM Temin, SA Aaronson |
| Vector： | Construct size (kb): 5.400000095367432 |
| Insert： | DNA: genomic Insert lengths(kb): 1.200000047683716 Gene product: platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)(platelet-derived growth factor, beta polypeptide) SIS Alleles: B1, B2, B1, B2 |
| Insert Size (kb)： | 1.200 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.0; PstI--4.8, 1.2. A 1.0 kb PstI/XbaI fragment contains v-sis but no helper virus sequences. Contains nt 4052-5122 of the published sequence, including most of coding sequence for v-sis, a polyadenylation signal, the v-sis helper virus junction, the coding sequence for p15E, and part of an LTR. |
| References： | Fabisiak JP, et al. Increased expression of PDGF-B (c-sis) mRNA in rat lung precedes DNA synthesis and tissue repair during chronic hypoxia. Am. J. Respir. Cell Mol. Biol. 1: 181-189, 1989. PubMed: 2696512 Radice P, et al. Loss of heterozygosity in human germinal tumors. Cytogenet. Cell Genet. 52: 72-76, 1989. PubMed: 2612217 Kochevar DT, et al. Low level amplification of c-sis and c-myc in a spontaneous osteosarcoma model. Cancer Lett. 53: 213-222, 1990. PubMed: 2208081 Robbins KC, et al. Molecular cloning of integrated simian sarcoma virus: genome organization of infectious DNA clones. Proc. Natl. Acad. Sci. USA 78: 2918-2922, 1981. PubMed: 6265924 Devare SG, et al. Nucleotide sequence of the simian sarcoma virus genome: demonstration that its acquired cellular sequences encode the transforming gene product p28sis. Proc. Natl. Acad. Sci. USA 80: 731-735, 1983. PubMed: 6298772 |
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| 产品名称： | pv-sis SISR12 |
|---|---|
| 商品货号： | TS194271 |
| Designations： | pv-sis SISR12 |
| GenBank Number： | J02396 |
| Species： | simian sarcoma virus (type C) |
| Depositors： | HM Temin, SA Aaronson |
| Vector： | Construct size (kb): 5.400000095367432 |
| Insert： | DNA: genomic Insert lengths(kb): 1.200000047683716 Gene product: platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)(platelet-derived growth factor, beta polypeptide) SIS Alleles: B1, B2, B1, B2 |
| Insert Size (kb)： | 1.200 |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.0; PstI--4.8, 1.2. A 1.0 kb PstI/XbaI fragment contains v-sis but no helper virus sequences. Contains nt 4052-5122 of the published sequence, including most of coding sequence for v-sis, a polyadenylation signal, the v-sis helper virus junction, the coding sequence for p15E, and part of an LTR. |
| References： | Fabisiak JP, et al. Increased expression of PDGF-B (c-sis) mRNA in rat lung precedes DNA synthesis and tissue repair during chronic hypoxia. Am. J. Respir. Cell Mol. Biol. 1: 181-189, 1989. PubMed: 2696512 Radice P, et al. Loss of heterozygosity in human germinal tumors. Cytogenet. Cell Genet. 52: 72-76, 1989. PubMed: 2612217 Kochevar DT, et al. Low level amplification of c-sis and c-myc in a spontaneous osteosarcoma model. Cancer Lett. 53: 213-222, 1990. PubMed: 2208081 Robbins KC, et al. Molecular cloning of integrated simian sarcoma virus: genome organization of infectious DNA clones. Proc. Natl. Acad. Sci. USA 78: 2918-2922, 1981. PubMed: 6265924 Devare SG, et al. Nucleotide sequence of the simian sarcoma virus genome: demonstration that its acquired cellular sequences encode the transforming gene product p28sis. Proc. Natl. Acad. Sci. USA 80: 731-735, 1983. PubMed: 6298772 |
