你好，请登录   免费注册    |    收藏本站
联系电话: 0574-87917803
你好,请登录   免费注册 | 收藏
联系电话: call_new 0574-87917803
热门搜索: 金黄色葡萄球菌 铜绿假单胞菌 红菇 香菇
Leishmania venezuelensis
Leishmania venezuelensis
规格:
货期:
编号:TS195183
品牌:Testobio
产品名称： Leishmania venezuelensis
商品货号： TS195183
Strain Designations： MHOM/VE/80/H-16
Biosafety Level： 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation：
skin ulcer from human with cutaneous leishmaniasis, Venezuela, 1980
(JA ODaly, personal communication )
Product Format： frozen
Storage Conditions： Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Axenic/Xenic： Axenic
Type Strain： no
Comments：
Experimental testing of antigens in vaccines and clinical psoriasis studies
RefODaly JA, et al. Antigens from Leishmania amastigotes induced clinical remission of psoriasis. Arch. Dermatol. Res. 301: 1-13, 2009. PubMed: 18777031RefODaly JA, et al. Isolation of Leishmania amastigote protein fractions which induced lymphocyte stimulation and remission of psoriasis. Arch. Dermatol. Res. 301: 411-427, 2009. PubMed: 19294395
Medium： ATCC® Medium 2736: M199, Modified Medium
ATCC® Medium 1011: Diphasic blood agar medium
ATCC® Medium 807: Brain heart infusion blood agar
Growth Conditions：
Temperature: 20-25°C
Cryopreservation：
  1. Harvest cells from a culture which is at or near peak density by centrifugation at ~800 x g for 5 min.
  2. Adjust concentration of cells to 2 x 107/ml in fresh medium. xa0If the concentration is too low, centrifuge at ~800 x g for 5 minutes and resuspend the cell pellet with a volume of supernatant to yield the desired concentration.
  3. While cells are centrifuging, prepare a 10% (v/v) solution of sterile DMSO in fresh medium (broth).xa0 The DMSO solution when first prepared will warm up due to chemical heat. The solution should be allowed to return to room temperature prior to use.
  4. Mix the cell preparation and the DMSO solution in equal portions. The final concentration will be 107 cells/ml and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no less than 15 min and no more than 30 min.
  5. Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place the ampules in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.)xa0 If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion.xa0 At -40°C, plunge ampules into liquid nitrogen.
  7. Store in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes.xa0 Do not agitate the ampule.xa0 Do not leave ampule in water bath after it is thawed.
  9. xa0Remove the vial from the water bath immediately after thawing.xa0 Aseptically transfer the contents of the ampule into 10 ml of fresh ATCC medium 2736.xa0
  10. Incubate the flask at 20-25°C with the cap screwed on tightly.
  11. Maintain as described above.
Name of Depositor： JA ODaly
Chain of Custody：
JA ODaly
References：

ODaly JA, et al. Antigens from Leishmania amastigotes induced clinical remission of psoriasis. Arch. Dermatol. Res. 301: 1-13, 2009. PubMed: 18777031

ODaly JA, et al. Isolation of Leishmania amastigote protein fractions which induced lymphocyte stimulation and remission of psoriasis. Arch. Dermatol. Res. 301: 411-427, 2009. PubMed: 19294395

JA ODaly, personal communication

首页 > 产品中心 > 微生物培养 > 菌株 > null > Leishmania venezuelensis

产品中心 Product Center

联系我们CONTACT US

  • 0574-87917803
    24小时服务热线
  • testobio@163.com
    销售邮箱
  • 浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
    地址

联系方式

  • 章丽敏
    17757489010
  • 朱静
    18067106081
  • 李晴
    18067408036
  • 李晶晶
    13375187189
  • 周雪
    13372109301

Leishmania venezuelensis

  • 货号: TS195183
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称： Leishmania venezuelensis
商品货号： TS195183
Strain Designations： MHOM/VE/80/H-16
Biosafety Level： 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation：
skin ulcer from human with cutaneous leishmaniasis, Venezuela, 1980
(JA ODaly, personal communication )
Product Format： frozen
Storage Conditions： Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Axenic/Xenic： Axenic
Type Strain： no
Comments：
Experimental testing of antigens in vaccines and clinical psoriasis studies
RefODaly JA, et al. Antigens from Leishmania amastigotes induced clinical remission of psoriasis. Arch. Dermatol. Res. 301: 1-13, 2009. PubMed: 18777031RefODaly JA, et al. Isolation of Leishmania amastigote protein fractions which induced lymphocyte stimulation and remission of psoriasis. Arch. Dermatol. Res. 301: 411-427, 2009. PubMed: 19294395
Medium： ATCC® Medium 2736: M199, Modified Medium
ATCC® Medium 1011: Diphasic blood agar medium
ATCC® Medium 807: Brain heart infusion blood agar
Growth Conditions：
Temperature: 20-25°C
Cryopreservation：
  1. Harvest cells from a culture which is at or near peak density by centrifugation at ~800 x g for 5 min.
  2. Adjust concentration of cells to 2 x 107/ml in fresh medium. xa0If the concentration is too low, centrifuge at ~800 x g for 5 minutes and resuspend the cell pellet with a volume of supernatant to yield the desired concentration.
  3. While cells are centrifuging, prepare a 10% (v/v) solution of sterile DMSO in fresh medium (broth).xa0 The DMSO solution when first prepared will warm up due to chemical heat. The solution should be allowed to return to room temperature prior to use.
  4. Mix the cell preparation and the DMSO solution in equal portions. The final concentration will be 107 cells/ml and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no less than 15 min and no more than 30 min.
  5. Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place the ampules in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.)xa0 If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion.xa0 At -40°C, plunge ampules into liquid nitrogen.
  7. Store in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes.xa0 Do not agitate the ampule.xa0 Do not leave ampule in water bath after it is thawed.
  9. xa0Remove the vial from the water bath immediately after thawing.xa0 Aseptically transfer the contents of the ampule into 10 ml of fresh ATCC medium 2736.xa0
  10. Incubate the flask at 20-25°C with the cap screwed on tightly.
  11. Maintain as described above.
Name of Depositor： JA ODaly
Chain of Custody：
JA ODaly
References：

ODaly JA, et al. Antigens from Leishmania amastigotes induced clinical remission of psoriasis. Arch. Dermatol. Res. 301: 1-13, 2009. PubMed: 18777031

ODaly JA, et al. Isolation of Leishmania amastigote protein fractions which induced lymphocyte stimulation and remission of psoriasis. Arch. Dermatol. Res. 301: 411-427, 2009. PubMed: 19294395

JA ODaly, personal communication

testopic
小红书
扫码关注小红书账号
关注我们
联系电话：0574-87917803
章丽敏：17757489010
朱静：18067106081
李晴：18067408036
李晶晶：13375187189
周雪：13372109301
邮箱：testobio@163.com
地址：浙江省宁波市高新区晶辉路866弄33号智造港E4幢3楼
在线留言
合作单位: