pRS166
pRS166
规格:
货期:
编号:TS195232
品牌:Testobio
| 产品名称： | pRS166 |
|---|---|
| 商品货号： | TS195232 |
| Designations： | pRS166 |
| Depositors： | P Hieter |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.3000001907348630 Vector: pRS166 (phagemid) Promoters: Promoter T3 Construction: pRSS56 pBluescript KS+, pBS(+), GAL1/10 promoter Marker(s):TRP1,ampR Construct size (kb): 6.300000190734863 Features: insert detection: lacZ marker(s): ampR, TRP1 promoter: lac, T3, T7, GAL10, URA3 replicon: pMB1, f1, ARS1 |
| Applications： | YC-type (centromeric) shuttle vector YX-type (expression) shuttle vector expression vector shuttle vector vector containing primer sites useful for sequencing vector permitting RNA synthesis in vitro vector permitting construction of fusion proteins vector permitting production of single-stranded DNA vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): PvuI--3.6, 3.0; SalI--6.6; EcoRI--6.6; SmaI--6.6; BamHI--6.6. Expression is inducible by galactose. YX-type expression shuttle vector permitting visual detection of recombinants and production of ssDNA in E. coli. Contains promoters for in vitro RNA synthesis and encodes the lacZ alpha (lacZ) peptide. Transcription from the GAL10 promoter (ATG is included in the sequence) permits the synthesis of a fusion protein (starting with 26 aa of GAL10) through the polylinker in the same frame as lacZ. pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+). A fragment containing the TRP1 gene was inserted into the NdeI site, a DraIII/EcoRI fragment containing the GAL10 promoter was inserted into the Asp718 site and a URA3 fragment was inserted between the SpeI and NotI sites of the MCS. The order of the major features in this plasmid is: TRP1 - URA 3 - f1 ori (NaeI) - T7 promoter - lacZ/MCS - T3 promoter - GAL10 promoter - GAL1 promoter - pMB1 ori - bla - CEN - ARS1. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Philip Hieter, personal communication |
| Related Products： | component of:ATCC 87054 |
| Shipped： | freeze-dried |
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| 产品名称： | pRS166 |
|---|---|
| 商品货号： | TS195232 |
| Designations： | pRS166 |
| Depositors： | P Hieter |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.3000001907348630 Vector: pRS166 (phagemid) Promoters: Promoter T3 Construction: pRSS56 pBluescript KS+, pBS(+), GAL1/10 promoter Marker(s):TRP1,ampR Construct size (kb): 6.300000190734863 Features: insert detection: lacZ marker(s): ampR, TRP1 promoter: lac, T3, T7, GAL10, URA3 replicon: pMB1, f1, ARS1 |
| Applications： | YC-type (centromeric) shuttle vector YX-type (expression) shuttle vector expression vector shuttle vector vector containing primer sites useful for sequencing vector permitting RNA synthesis in vitro vector permitting construction of fusion proteins vector permitting production of single-stranded DNA vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): PvuI--3.6, 3.0; SalI--6.6; EcoRI--6.6; SmaI--6.6; BamHI--6.6. Expression is inducible by galactose. YX-type expression shuttle vector permitting visual detection of recombinants and production of ssDNA in E. coli. Contains promoters for in vitro RNA synthesis and encodes the lacZ alpha (lacZ) peptide. Transcription from the GAL10 promoter (ATG is included in the sequence) permits the synthesis of a fusion protein (starting with 26 aa of GAL10) through the polylinker in the same frame as lacZ. pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+). A fragment containing the TRP1 gene was inserted into the NdeI site, a DraIII/EcoRI fragment containing the GAL10 promoter was inserted into the Asp718 site and a URA3 fragment was inserted between the SpeI and NotI sites of the MCS. The order of the major features in this plasmid is: TRP1 - URA 3 - f1 ori (NaeI) - T7 promoter - lacZ/MCS - T3 promoter - GAL10 promoter - GAL1 promoter - pMB1 ori - bla - CEN - ARS1. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Philip Hieter, personal communication |
| Related Products： | component of:ATCC 87054 |
| Shipped： | freeze-dried |
