| 产品名称： | pCAS4 NEB681 |
|---|---|
| 商品货号： | TS195758 |
| Designations： | pCAS4 NEB681 |
| GenBank Number： | M74198 |
| Species： | Thermococcus litoralis Neuner et al. |
| Depositors： | New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc. |
| Applications： | produces protein DNA polymerase (Vent) |
| Vector： | Construct size (kb): 10.5 |
| Insert： | DNA: genomic Insert lengths(kb): 3.799999952316284 Gene product: DNA polymerase (Vent) pol Target Gene: DNA polymerase (Vent) |
| Insert Size (kb)： | 3.800 |
| Media： | ATCC® Medium 1949: LB medium (ATCC medium 1065) with 50 mcg/ml chloramphenicol and 100 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of pCAS4 give the following sizes (kb): BamHI--10.5; EcoRI--9.0, 1.9; HindIII--9.0, 0.7, 0.7. pLysS is also detected at 5.1 kb with EcoRI or HindIII, or 4.4 and 0.6 with BamHI. Restriction digest of the pCAS4 gives the following sizes (kb): EcoRV--8.2,1.9. The insert differs from that of NEB671 (ATCC 68447) by the modification the sequence encoding aa 141-143 (GATATTGAA) to GCGATCGCA. This changes the aa sequence of the protein from DIE to AIA and inactivates 3 to 5 exonuclease activity. |
| References： | Comb DG, et al. Purified thermostable DNA polymerase obtainable from Thermococcus litoralis. US Patent 5,322,785 dated Jun 21 1994 Comb DG, et al. Recombinant thermostable DNA polymerase from archaebacteria. US Patent 5,352,778 dated Oct 4 1994 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pCAS4 NEB681 |
|---|---|
| 商品货号： | TS195758 |
| Designations： | pCAS4 NEB681 |
| GenBank Number： | M74198 |
| Species： | Thermococcus litoralis Neuner et al. |
| Depositors： | New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc. |
| Applications： | produces protein DNA polymerase (Vent) |
| Vector： | Construct size (kb): 10.5 |
| Insert： | DNA: genomic Insert lengths(kb): 3.799999952316284 Gene product: DNA polymerase (Vent) pol Target Gene: DNA polymerase (Vent) |
| Insert Size (kb)： | 3.800 |
| Media： | ATCC® Medium 1949: LB medium (ATCC medium 1065) with 50 mcg/ml chloramphenicol and 100 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of pCAS4 give the following sizes (kb): BamHI--10.5; EcoRI--9.0, 1.9; HindIII--9.0, 0.7, 0.7. pLysS is also detected at 5.1 kb with EcoRI or HindIII, or 4.4 and 0.6 with BamHI. Restriction digest of the pCAS4 gives the following sizes (kb): EcoRV--8.2,1.9. The insert differs from that of NEB671 (ATCC 68447) by the modification the sequence encoding aa 141-143 (GATATTGAA) to GCGATCGCA. This changes the aa sequence of the protein from DIE to AIA and inactivates 3 to 5 exonuclease activity. |
| References： | Comb DG, et al. Purified thermostable DNA polymerase obtainable from Thermococcus litoralis. US Patent 5,322,785 dated Jun 21 1994 Comb DG, et al. Recombinant thermostable DNA polymerase from archaebacteria. US Patent 5,352,778 dated Oct 4 1994 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
