| 产品名称： | LYD9 plasmid in E. coli |
|---|---|
| 商品货号： | TS196058 |
| Designations： | LYD9 plasmid in E. coli |
| Species： | lethal yellowing phytoplasma |
| Depositors： | NA Harrison |
| Vector： | Construct size (kb): 6.80 |
| Insert： | DNA: genomic Insert lengths(kb): 4.1 Gene product: DNA Segment |
| Insert Size (kb)： | 4.100 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.1, 2.7; BamHI--6.8; HindIII--3.9, 1.55, 1.3; PstI--6.8; SalI--6.8. DNA of the lethal yellowing agent was isolated from Manila palm (Veitchia merrillii). The extent to which the lethal yellowing DNA probes hybridize to other mollicute DNAs varies according to the hybridization conditions. Hybridizes broadly to DNA of Florida MLOs. Also detects African coconut lethal decline agents associated with lethal disease (Tanzania) and Awka disease (Nigeria). Also hybridizes to acholeplasma but not to spiroplasma DNA. |
| References： | Harrison NA, et al. DNA probes for detection of mycoplasmalike organisms associated with lethal yellowing disease of palms in Florida. Mol. Plant Pathol. 82: 216-224, 1992. Nigel A Harrison, personal communication |
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| 产品名称： | LYD9 plasmid in E. coli |
|---|---|
| 商品货号： | TS196058 |
| Designations： | LYD9 plasmid in E. coli |
| Species： | lethal yellowing phytoplasma |
| Depositors： | NA Harrison |
| Vector： | Construct size (kb): 6.80 |
| Insert： | DNA: genomic Insert lengths(kb): 4.1 Gene product: DNA Segment |
| Insert Size (kb)： | 4.100 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.1, 2.7; BamHI--6.8; HindIII--3.9, 1.55, 1.3; PstI--6.8; SalI--6.8. DNA of the lethal yellowing agent was isolated from Manila palm (Veitchia merrillii). The extent to which the lethal yellowing DNA probes hybridize to other mollicute DNAs varies according to the hybridization conditions. Hybridizes broadly to DNA of Florida MLOs. Also detects African coconut lethal decline agents associated with lethal disease (Tanzania) and Awka disease (Nigeria). Also hybridizes to acholeplasma but not to spiroplasma DNA. |
| References： | Harrison NA, et al. DNA probes for detection of mycoplasmalike organisms associated with lethal yellowing disease of palms in Florida. Mol. Plant Pathol. 82: 216-224, 1992. Nigel A Harrison, personal communication |
