| 产品名称： | pEAD |
|---|---|
| 商品货号： | TS196191 |
| Designations： | pEAD |
| GenBank Number： | V01555 |
| Species： | Human gammaherpesvirus 4 (HHV-4), Epstein-Barr virus |
| Depositors： | Johns Hopkins University, G Milman, Johns Hopkins University |
| Applications： | produces protein early antigen, diffuse (open reading frame BMRF1) |
| Vector： | Construct size (kb): 8.34000015258789 |
| Insert： | DNA: genomic Insert lengths(kb): 4.340000152587891 Tissue: B95-8 cell line Gene product: early antigen, diffuse (open reading frame BMRF1) EBEAD Target Gene: early antigen, diffuse (open reading frame BMRF1) |
| Insert Size (kb)： | 4.340 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII--6.6, 1.6; EcoRI--8.2; XbaI--8.2. The insert contains the following restriction sites (approximate kb from the 5 end): BglII--1.34; EcoRI--3.03; HindIII--2.19; KpnI--1.42; NotI--0.91; PstI--0.10, 3.05, 4.23; SacI--3.78; SmaI--0.95, 1.82, 3.84; XbaI--1.96; XhoI--1.79, 3.73. pHE6 is a high-expression plasmid derived from pUC8. Inserts are expressed as run-on products initiated at lambda N. Synthesis is regulated by the cI857 repressor encoded by the plasmid. Corresponds to nt 79895-84233 of the GenBank record. Expresses a 47 kDa fusion polypeptide composed of 33 amino acids from the lambda N protein and the BMRF1 open reading frame. This is recognized by monoclonal antibody R3. |
| Classification： | Herpesviridae, Lymphocryptovirus |
| References： | Milman G. Expression of antigenic epstein-barr virus polypeptides in bacteria and their use in diagnostics. US Patent 5,256,768 dated Oct 26 1993 Cho MS, et al. A second Epstein-Barr virus early antigen gene in BamHI fragment M encodes a 48- to 50-kilodalton nuclear protein. J. Virol. 56: 860-866, 1985. PubMed: 2999442 Baer R, et al. DNA sequence and expression of the B95-8 Epstein-Barr virus genome. Nature 310: 207-211, 1984. PubMed: 6087149 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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| 产品名称： | pEAD |
|---|---|
| 商品货号： | TS196191 |
| Designations： | pEAD |
| GenBank Number： | V01555 |
| Species： | Human gammaherpesvirus 4 (HHV-4), Epstein-Barr virus |
| Depositors： | Johns Hopkins University, G Milman, Johns Hopkins University |
| Applications： | produces protein early antigen, diffuse (open reading frame BMRF1) |
| Vector： | Construct size (kb): 8.34000015258789 |
| Insert： | DNA: genomic Insert lengths(kb): 4.340000152587891 Tissue: B95-8 cell line Gene product: early antigen, diffuse (open reading frame BMRF1) EBEAD Target Gene: early antigen, diffuse (open reading frame BMRF1) |
| Insert Size (kb)： | 4.340 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglII--6.6, 1.6; EcoRI--8.2; XbaI--8.2. The insert contains the following restriction sites (approximate kb from the 5 end): BglII--1.34; EcoRI--3.03; HindIII--2.19; KpnI--1.42; NotI--0.91; PstI--0.10, 3.05, 4.23; SacI--3.78; SmaI--0.95, 1.82, 3.84; XbaI--1.96; XhoI--1.79, 3.73. pHE6 is a high-expression plasmid derived from pUC8. Inserts are expressed as run-on products initiated at lambda N. Synthesis is regulated by the cI857 repressor encoded by the plasmid. Corresponds to nt 79895-84233 of the GenBank record. Expresses a 47 kDa fusion polypeptide composed of 33 amino acids from the lambda N protein and the BMRF1 open reading frame. This is recognized by monoclonal antibody R3. |
| Classification： | Herpesviridae, Lymphocryptovirus |
| References： | Milman G. Expression of antigenic epstein-barr virus polypeptides in bacteria and their use in diagnostics. US Patent 5,256,768 dated Oct 26 1993 Cho MS, et al. A second Epstein-Barr virus early antigen gene in BamHI fragment M encodes a 48- to 50-kilodalton nuclear protein. J. Virol. 56: 860-866, 1985. PubMed: 2999442 Baer R, et al. DNA sequence and expression of the B95-8 Epstein-Barr virus genome. Nature 310: 207-211, 1984. PubMed: 6087149 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
