| 产品名称： | BSC40 BSC-40 |
|---|---|
| 商品货号： | TS196376 |
| Organism： | Cercopithecus aethiops |
| Tissue： | kidney |
| Cell Type： | epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | This cell line is a suitable viral host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | BSC40 is a high-temperature tolerant derivative of BS-C-1 established by Brockman and Nathans. |
| Virus Susceptibility： | Vaccinia virus
|
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:5 to 1:10 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells: a Manual of Basic Technique by R. Ian Freshney, 5th edition, published by Alan R. Liss, N.Y., 1994.
|
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Max Temperature: 40°C
Min Temperature: 31°C |
| Name of Depositor： | RC Condit |
| References： | Condit RC, Motyczka A. Isolation and preliminary characterization of temperature-sensitive mutants of vaccinia virus. Virology 113: 224-241, 1981. PubMed: 7269240 Brockman WW, Nathans D. The isolation of simian virus 40 variants with specifically altered genomes. Proc. Natl. Acad. Sci. USA 71: 942-946, 1974. PubMed: 4362640 Ensinger MJ. Isolation and genetic characterization of temperature-sensitive mutants of vaccinia virus WR. J. Virol. 43: 778-790, 1982. PubMed: 7143565 BSC40 is a high-temperature tolerant derivative of BS-C-1 established by Brockman and Nathans. BSC40 cells may be grown at temperature from 31C to 40C. The cells are useful for selection and assay of temperature sensitive viruses. They support the growth of vaccinia virus, and can be used as a host for vaccinia virus genetic studies. |
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| 产品名称： | BSC40 BSC-40 |
|---|---|
| 商品货号： | TS196376 |
| Organism： | Cercopithecus aethiops |
| Tissue： | kidney |
| Cell Type： | epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | This cell line is a suitable viral host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | BSC40 is a high-temperature tolerant derivative of BS-C-1 established by Brockman and Nathans. |
| Virus Susceptibility： | Vaccinia virus
|
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:5 to 1:10 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells: a Manual of Basic Technique by R. Ian Freshney, 5th edition, published by Alan R. Liss, N.Y., 1994.
|
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Max Temperature: 40°C
Min Temperature: 31°C |
| Name of Depositor： | RC Condit |
| References： | Condit RC, Motyczka A. Isolation and preliminary characterization of temperature-sensitive mutants of vaccinia virus. Virology 113: 224-241, 1981. PubMed: 7269240 Brockman WW, Nathans D. The isolation of simian virus 40 variants with specifically altered genomes. Proc. Natl. Acad. Sci. USA 71: 942-946, 1974. PubMed: 4362640 Ensinger MJ. Isolation and genetic characterization of temperature-sensitive mutants of vaccinia virus WR. J. Virol. 43: 778-790, 1982. PubMed: 7143565 BSC40 is a high-temperature tolerant derivative of BS-C-1 established by Brockman and Nathans. BSC40 cells may be grown at temperature from 31C to 40C. The cells are useful for selection and assay of temperature sensitive viruses. They support the growth of vaccinia virus, and can be used as a host for vaccinia virus genetic studies. |
