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Helicosporidium sp.
Helicosporidium sp.
规格:
货期:
编号:TS196652
品牌:Testobio
产品名称： Helicosporidium sp.
商品货号： TS196652
Strain Designations： Dm-1
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： Dendroctonus micans, whole insect homogenate, Turkey, 2004
Product Format： frozen
Comments： Insect pathogen
Medium： ATCC® Medium 28: Emmons modification of Sabourauds agar
Growth Conditions： Temperature: 25°C
Cryopreservation：
  1. Harvest cells from a culture which is at or near peak density by centrifugation at ~800 x g for 5 min.
  2. Adjust concentration of cells to 2 x 107/mL in fresh medium. xa0If the concentration is too low, centrifuge at ~800 x g for 5 minutes and resuspend the cell pellet with a volume of supernatant to yield the desired concentration.
  3. While cells are centrifuging, prepare a 20% (v/v) solution of sterile DMSO in fresh medium (broth).xa0 The DMSO solution when first prepared will warm up due to chemical heat. The solution should be allowed to return to room temperature prior to use.
  4. Mix the cell preparation and the DMSO solution in equal portions. The final concentration will be 107 cells/mL and 10% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no less than 15 min and no more than 30 min.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place the ampules in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.)xa0 If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion.xa0 At -40°C, plunge ampules into liquid nitrogen.
  7. Store in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes.xa0 Do not agitate the ampule.xa0 Do not leave ampule in water bath after it is thawed.
  9. Remove the vial from the water bath immediately after thawing.xa0 Aseptically transfer the contents of the ampule into 10 mL of fresh ATCC medium 28.xa0
  10. Incubate the flask at 25°C with the cap screwed on tightly.
  11. Maintain as described above.
Name of Depositor： D Boucias
Cross References：

Nucleotide (GenBank) : JN869308 Beta-tubulin

Nucleotide (GenBank) : JN869301 18S rRNA gene

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Helicosporidium sp.

  • 货号: TS196652
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称： Helicosporidium sp.
商品货号： TS196652
Strain Designations： Dm-1
Biosafety Level： 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation： Dendroctonus micans, whole insect homogenate, Turkey, 2004
Product Format： frozen
Comments： Insect pathogen
Medium： ATCC® Medium 28: Emmons modification of Sabourauds agar
Growth Conditions： Temperature: 25°C
Cryopreservation：
  1. Harvest cells from a culture which is at or near peak density by centrifugation at ~800 x g for 5 min.
  2. Adjust concentration of cells to 2 x 107/mL in fresh medium. xa0If the concentration is too low, centrifuge at ~800 x g for 5 minutes and resuspend the cell pellet with a volume of supernatant to yield the desired concentration.
  3. While cells are centrifuging, prepare a 20% (v/v) solution of sterile DMSO in fresh medium (broth).xa0 The DMSO solution when first prepared will warm up due to chemical heat. The solution should be allowed to return to room temperature prior to use.
  4. Mix the cell preparation and the DMSO solution in equal portions. The final concentration will be 107 cells/mL and 10% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no less than 15 min and no more than 30 min.
  5. Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
  6. Place the ampules in a Nalgene 1°C freezing apparatus.xa0 Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen.xa0 (The cooling rate in this apparatus is approximately -1°C/min.)xa0 If freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through heat of fusion.xa0 At -40°C, plunge ampules into liquid nitrogen.
  7. Store in either the vapor or liquid phase of a nitrogen refrigerator.
  8. To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes.xa0 Do not agitate the ampule.xa0 Do not leave ampule in water bath after it is thawed.
  9. Remove the vial from the water bath immediately after thawing.xa0 Aseptically transfer the contents of the ampule into 10 mL of fresh ATCC medium 28.xa0
  10. Incubate the flask at 25°C with the cap screwed on tightly.
  11. Maintain as described above.
Name of Depositor： D Boucias
Cross References：

Nucleotide (GenBank) : JN869308 Beta-tubulin

Nucleotide (GenBank) : JN869301 18S rRNA gene

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