| 产品名称： | Naegleria minor (Dobson et al.) De Jonckheere and Brown |
|---|---|
| 商品货号： | TS196998 |
| Deposited As： | Willaertia minor Dobson et al. |
| Strain Designations： | SWL WT-043 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | freshwater sediment, Berry Springs, Northern Territory, Australia, 1990 |
| Product Format： | dried |
| Type Strain： | no |
| Medium： | ATCC® Medium 997: Fresh water ameba medium |
| Growth Conditions： | Temperature: 25.0°C Duration: grown with Escherichia coli ATCC 11775 Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days. |
| Subcultivation： | Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days. |
| Cryopreservation： | 1. xa0 Allow the cells to encyst.xa0 To detach cysts from the plate flush the surface with 5 ml fresh ATCC medium 1323 (Pages Balanced Salt Solution).xa0 Rub the surface of the plate with a spread bar to detach adhering amoebae. 2.xa0xa0 Transfer the cyst suspension to a sterile centrifuge tube. 3. xa0 If the cyst concentration does not exceed 2 x 106 cysts/ml adjust the suspension to that concentration.xa0 To adjust the concentration, centrifuge at 600 x g for 5 min and resuspend the pellet in the volume of fresh medium required to yield 2 x 106. 4.xa0xa0 While cells are centrifuging prepare a 15% (v/v) solution of sterile DMSO as follows:xa0 Add the required volume of DMSO to a glass screw-capped test tube and place it in an ice bath.xa0 Allow the DMSO to solidify.xa0 Add the required volume of refrigerated medium.xa0 Dissolve the DMSO by inverting the tube several times.xa0 |
| Name of Depositor： | BS Robinson |
| Year of Origin： | 1990 |
| References： | De Jonckheere JF. A century of research on the amoeboflagellate genus Naegleria. Acta Protozool. 41: 309-342, 2002. |
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| 产品名称： | Naegleria minor (Dobson et al.) De Jonckheere and Brown |
|---|---|
| 商品货号： | TS196998 |
| Deposited As： | Willaertia minor Dobson et al. |
| Strain Designations： | SWL WT-043 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | freshwater sediment, Berry Springs, Northern Territory, Australia, 1990 |
| Product Format： | dried |
| Type Strain： | no |
| Medium： | ATCC® Medium 997: Fresh water ameba medium |
| Growth Conditions： | Temperature: 25.0°C Duration: grown with Escherichia coli ATCC 11775 Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days. |
| Subcultivation： | Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days. |
| Cryopreservation： | 1. xa0 Allow the cells to encyst.xa0 To detach cysts from the plate flush the surface with 5 ml fresh ATCC medium 1323 (Pages Balanced Salt Solution).xa0 Rub the surface of the plate with a spread bar to detach adhering amoebae. 2.xa0xa0 Transfer the cyst suspension to a sterile centrifuge tube. 3. xa0 If the cyst concentration does not exceed 2 x 106 cysts/ml adjust the suspension to that concentration.xa0 To adjust the concentration, centrifuge at 600 x g for 5 min and resuspend the pellet in the volume of fresh medium required to yield 2 x 106. 4.xa0xa0 While cells are centrifuging prepare a 15% (v/v) solution of sterile DMSO as follows:xa0 Add the required volume of DMSO to a glass screw-capped test tube and place it in an ice bath.xa0 Allow the DMSO to solidify.xa0 Add the required volume of refrigerated medium.xa0 Dissolve the DMSO by inverting the tube several times.xa0 |
| Name of Depositor： | BS Robinson |
| Year of Origin： | 1990 |
| References： | De Jonckheere JF. A century of research on the amoeboflagellate genus Naegleria. Acta Protozool. 41: 309-342, 2002. |
