| 产品名称： | CD70 CD70.1 |
|---|---|
| 商品货号： | TS197411 |
| Designations： | CD70 CD70.1 |
| GenBank Number： | L08096 |
| Species： | Homo sapiens, human |
| Applications： | produces protein CD70 antigen (CD27 ligand) |
| Vector： | Construct size (kb): 5.099999904632568 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pMT2 Intact vector size: 4.200 Type of vector: plasmid Vector end: BstXI Vector end: BstXI Cloning sites: EcoRI Polylinker sites: Construction: pBR322; Ad VAI; Ad VAII; Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): ampR other: non-coding DHFR promoter: adenovirus major late replicon: pMB1, SV40 terminator: SV4O Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CD70 Other gene symbols: CD70.1; CD27LG; CD27L Gene name: CD70 antigen (CD27 ligand) Contains complete coding sequence?: Y Chromosome: 19; Localization: 19 p13 Tissue: B-lymphocyte JY cell line, EBV transformed Type of DNA: cDNA Insert end: Modification: BstXI adapt Insert end: Modification: BstXI adapt Insert size (kb): 0.89 Cross references: DNA Seq. Acc.: L08096 Nucleotides 6-888 of the insert correspond to nucleotides 19-901 of L08096. Insert lengths(kb): 0.8899999856948853 Tissue: B lymphocyte (EBV transformed), JY cell line Gene product: CD70 antigen (CD27 ligand) CD70 Target Gene: CD70 antigen (CD27 ligand) |
| Insert Size (kb)： | 0.890 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): ApaI--5.4; EcoRI--4.4, 0.96; NheI--3.8, 1.6; SmaI--4.6, 0.76; XhoI--5.4. The insert contains the following restrictions sites (approximate kb from the 5 end): SmaI--0.05, 0.80; NheI--0.11; XhoI--0.29; ApaI--0.37. |
| References： | Bowman MR, et al. The cloning of CD70 and its identification as the ligand for CD27. J. Immunol. 152: 1756-1761, 1994. PubMed: 8120384 Goodwin RG, et al. Molecular and biological characterization of a ligand for CD27 defines a new family of cytokines with homology to tumor necrosis factor. Cell 73: 447-456, 1993. PubMed: 8387892 |
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| 产品名称： | CD70 CD70.1 |
|---|---|
| 商品货号： | TS197411 |
| Designations： | CD70 CD70.1 |
| GenBank Number： | L08096 |
| Species： | Homo sapiens, human |
| Applications： | produces protein CD70 antigen (CD27 ligand) |
| Vector： | Construct size (kb): 5.099999904632568 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pMT2 Intact vector size: 4.200 Type of vector: plasmid Vector end: BstXI Vector end: BstXI Cloning sites: EcoRI Polylinker sites: Construction: pBR322; Ad VAI; Ad VAII; Host range: vertebrate cells; Escherichia coli Features (with orientation and position when available): marker(s): ampR other: non-coding DHFR promoter: adenovirus major late replicon: pMB1, SV40 terminator: SV4O Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: CD70 Other gene symbols: CD70.1; CD27LG; CD27L Gene name: CD70 antigen (CD27 ligand) Contains complete coding sequence?: Y Chromosome: 19; Localization: 19 p13 Tissue: B-lymphocyte JY cell line, EBV transformed Type of DNA: cDNA Insert end: Modification: BstXI adapt Insert end: Modification: BstXI adapt Insert size (kb): 0.89 Cross references: DNA Seq. Acc.: L08096 Nucleotides 6-888 of the insert correspond to nucleotides 19-901 of L08096. Insert lengths(kb): 0.8899999856948853 Tissue: B lymphocyte (EBV transformed), JY cell line Gene product: CD70 antigen (CD27 ligand) CD70 Target Gene: CD70 antigen (CD27 ligand) |
| Insert Size (kb)： | 0.890 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): ApaI--5.4; EcoRI--4.4, 0.96; NheI--3.8, 1.6; SmaI--4.6, 0.76; XhoI--5.4. The insert contains the following restrictions sites (approximate kb from the 5 end): SmaI--0.05, 0.80; NheI--0.11; XhoI--0.29; ApaI--0.37. |
| References： | Bowman MR, et al. The cloning of CD70 and its identification as the ligand for CD27. J. Immunol. 152: 1756-1761, 1994. PubMed: 8120384 Goodwin RG, et al. Molecular and biological characterization of a ligand for CD27 defines a new family of cytokines with homology to tumor necrosis factor. Cell 73: 447-456, 1993. PubMed: 8387892 |
