| 产品名称： | SU-DHL-8 |
|---|---|
| 商品货号： | TS197850 |
| Organism： | Homo sapiens, human |
| Tissue： | lymph node; derived from metastatic site: peritoneal effusion |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast-like |
| Culture Properties： | suspension, grow as single cells and small clusters |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Large cell lymphoma |
| Age： | 59 years |
| Gender： | Male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Clinical Data： | 59 years Caucasian Male |
| Receptor Expression： | Fc (not expressed), IgMEAC (not expressed), IgGEA (not expressed), E (not expressed) |
| Genes Expressed： | Ig +; IgM-, IgG-, IgA-, IgD-, Lambda-, Kappa- |
| Comments： | The depositor states that this cell line is negative for Epstein-Barr Virus (EBV). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium. Subculture the cells when cell density is ~ 1.0 x 106 to 2.5 x 106 cells/mL for higher viability. An inoculum of 1.0 x 105 to 4.0 x 105 cells/mL is recommended. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:10 is recommended. Medium renewal: Every 2 to 4 days (depending on cell density) |
| Cryopreservation： | Storage temperature:xa0liquid nitrogen vapor phase Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% (v/v) DMSO |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | D5S818: 11, 13 D13S317: 11, 13 D7S820: 8 D16S539: 12 CSF1PO: 11, 12 vWA: 15, 19 THO1: 6, 9 TPOX: 8 Amelogenin: X |
| Year of Origin： | 1978 |
| References： | Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220 |
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| 产品名称： | SU-DHL-8 |
|---|---|
| 商品货号： | TS197850 |
| Organism： | Homo sapiens, human |
| Tissue： | lymph node; derived from metastatic site: peritoneal effusion |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast-like |
| Culture Properties： | suspension, grow as single cells and small clusters |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Large cell lymphoma |
| Age： | 59 years |
| Gender： | Male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： | |
| Clinical Data： | 59 years Caucasian Male |
| Receptor Expression： | Fc (not expressed), IgMEAC (not expressed), IgGEA (not expressed), E (not expressed) |
| Genes Expressed： | Ig +; IgM-, IgG-, IgA-, IgD-, Lambda-, Kappa- |
| Comments： | The depositor states that this cell line is negative for Epstein-Barr Virus (EBV). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by the addition of fresh medium. Subculture the cells when cell density is ~ 1.0 x 106 to 2.5 x 106 cells/mL for higher viability. An inoculum of 1.0 x 105 to 4.0 x 105 cells/mL is recommended. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:10 is recommended. Medium renewal: Every 2 to 4 days (depending on cell density) |
| Cryopreservation： | Storage temperature:xa0liquid nitrogen vapor phase Freeze medium: complete growth medium supplemented with an additional 20% fetal bovine serum and 10% (v/v) DMSO |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | D5S818: 11, 13 D13S317: 11, 13 D7S820: 8 D16S539: 12 CSF1PO: 11, 12 vWA: 15, 19 THO1: 6, 9 TPOX: 8 Amelogenin: X |
| Year of Origin： | 1978 |
| References： | Epstein AL, et al. Biology of the human malignant lymphomas. IV. Functional characterization of ten diffuse histiocytic lymphoma cell lines. Cancer. 42(5): 2379-2391, 1978. PubMed: 214220 |
