| 产品名称： | HHCPB41 |
|---|---|
| 商品货号： | TS198521 |
| Designations： | HHCPB41 |
| GenBank Number： | M78063 |
| Species： | Homo sapiens, human |
| Depositors： | JC Venter |
| Applications： | The predicted product size is (bp): 96. The observed product size(s) is(are) (bp): 95 (human). |
| Vector： | Construct size (kb): 5.150000095367432 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: D4S815E Genomic copy number: unique Gene name: DNA Segment, single copy, expressed Contains complete coding sequence?: U Sequence homology: Homo sapiens protein phosphatase 2A, regulatory polypeptide beta (55 kDa) Chromosome: 4; Localization: 4 16.3 distal Tissue: hippocampus, female, 2 years, oligo-dT and random primed (Stratagene 936205), subtracted Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 2.15 Cross references: DNA Seq. Acc.: M78063; EST: EST01650; Primer: M13 Forward Insert lengths(kb): 2.150000095367432 Tissue: hippocampus, female, 2 years, oligo-dT and random primed (Stratagene 936205), subtracted Gene product: protein phosphatase 2A, regulatory polypeptide beta (55 kDa) D4S815E |
| Insert Size (kb)： | 2.150 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.9, 0.25; PstI--3.4, 1.1, 0.66; BamHI--5.2. EST01650 can be detected in genomic DNA using the following oligonucleotides (5-3): GACGTGTACAGCACTTTCCA and TGGGAGCCACTTGATCTT. EST01650 matches GenBank accession HUMPROP2AB with 76.81% identity over the sequence range: 1-289. This sequence record is for human protein phosphatase 2A, regulatory polypeptide beta (55 kDa). The predicted product size is (bp): 96. The observed product size(s) is(are) (bp): 95 (human). |
| References： | Korenberg JR, et al. Toward a cDNA map of the human genome. Genomics 29: 364-370, 1995. PubMed: 8666383 Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749 Durkin AS, et al. Chromosomal assignment of human brain expressed sequence tags (ESTS) by analzying fluorescently-labeled PCR products from hybrid cell panels. Cytogenet. Cell Genet. 65: 86-91, 1994. PubMed: 8404072 Maglott DR, et al259 human brain expressed sequence tags (ESTs): chromosome localization, subregional assignment, and sequence analysisIn: Maglott DR, et alIdentification of transcribed sequencesNew YorkPlenum Presspp. 273-288, 1994 J C Venter, personal communication |
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| 产品名称： | HHCPB41 |
|---|---|
| 商品货号： | TS198521 |
| Designations： | HHCPB41 |
| GenBank Number： | M78063 |
| Species： | Homo sapiens, human |
| Depositors： | JC Venter |
| Applications： | The predicted product size is (bp): 96. The observed product size(s) is(are) (bp): 95 (human). |
| Vector： | Construct size (kb): 5.150000095367432 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: EcoRI Vector end: EcoRI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: D4S815E Genomic copy number: unique Gene name: DNA Segment, single copy, expressed Contains complete coding sequence?: U Sequence homology: Homo sapiens protein phosphatase 2A, regulatory polypeptide beta (55 kDa) Chromosome: 4; Localization: 4 16.3 distal Tissue: hippocampus, female, 2 years, oligo-dT and random primed (Stratagene 936205), subtracted Type of DNA: cDNA Insert end: Modification: EcoRI linkers Insert end: Modification: EcoRI linkers Insert size (kb): 2.15 Cross references: DNA Seq. Acc.: M78063; EST: EST01650; Primer: M13 Forward Insert lengths(kb): 2.150000095367432 Tissue: hippocampus, female, 2 years, oligo-dT and random primed (Stratagene 936205), subtracted Gene product: protein phosphatase 2A, regulatory polypeptide beta (55 kDa) D4S815E |
| Insert Size (kb)： | 2.150 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.9, 0.25; PstI--3.4, 1.1, 0.66; BamHI--5.2. EST01650 can be detected in genomic DNA using the following oligonucleotides (5-3): GACGTGTACAGCACTTTCCA and TGGGAGCCACTTGATCTT. EST01650 matches GenBank accession HUMPROP2AB with 76.81% identity over the sequence range: 1-289. This sequence record is for human protein phosphatase 2A, regulatory polypeptide beta (55 kDa). The predicted product size is (bp): 96. The observed product size(s) is(are) (bp): 95 (human). |
| References： | Korenberg JR, et al. Toward a cDNA map of the human genome. Genomics 29: 364-370, 1995. PubMed: 8666383 Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749 Durkin AS, et al. Chromosomal assignment of human brain expressed sequence tags (ESTS) by analzying fluorescently-labeled PCR products from hybrid cell panels. Cytogenet. Cell Genet. 65: 86-91, 1994. PubMed: 8404072 Maglott DR, et al259 human brain expressed sequence tags (ESTs): chromosome localization, subregional assignment, and sequence analysisIn: Maglott DR, et alIdentification of transcribed sequencesNew YorkPlenum Presspp. 273-288, 1994 J C Venter, personal communication |
