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pSV5-dhfr
pSV5-dhfr
规格:
货期:
编号:TS198530
品牌:Testobio
产品名称: pSV5-dhfr
商品货号: TS198530
Designations: pSV5-dhfr
Depositors: P Berg
Biosafety Level: 1
Vector Information:
Size (kb): 9.30
Vector: pSV5-dhfr (plasmid)
Promoters: Promoter SV40
Construction: pBR322, SV40, mouse
Marker(s):ampR,mtxR
Construct size (kb): 9.30
Features: marker(s): ampR, mtxR
other: polyoma large T antigen
promoter: SV40
replicon: pMB1
Applications:
expression vector
shuttle vector
Comments:
Confers selective advantage to mammalian cell lines grown in the presence of methotrexate. Selectable marker in dhfr- cell culture strains.
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions:
Temperature: 37.0°C
References:

Subramani S, et al. Expression of the mouse dihydrofolate reductase complementary deoxyribonucleic acid in simian virus 40 vectors. Mol. Cell. Biol. 1: 854-864, 1981. PubMed: 9279398

Shipped: freeze-dried
Shipping Information: Distributed: freeze-dried
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pSV5-dhfr

  • 货号: TS198530
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: pSV5-dhfr
商品货号: TS198530
Designations: pSV5-dhfr
Depositors: P Berg
Biosafety Level: 1
Vector Information:
Size (kb): 9.30
Vector: pSV5-dhfr (plasmid)
Promoters: Promoter SV40
Construction: pBR322, SV40, mouse
Marker(s):ampR,mtxR
Construct size (kb): 9.30
Features: marker(s): ampR, mtxR
other: polyoma large T antigen
promoter: SV40
replicon: pMB1
Applications:
expression vector
shuttle vector
Comments:
Confers selective advantage to mammalian cell lines grown in the presence of methotrexate. Selectable marker in dhfr- cell culture strains.
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions:
Temperature: 37.0°C
References:

Subramani S, et al. Expression of the mouse dihydrofolate reductase complementary deoxyribonucleic acid in simian virus 40 vectors. Mol. Cell. Biol. 1: 854-864, 1981. PubMed: 9279398

Shipped: freeze-dried
Shipping Information: Distributed: freeze-dried
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