| 产品名称： | pGEM-hJE34 |
|---|---|
| 商品货号： | TS198632 |
| Designations： | pGEM-hJE34 |
| GenBank Number： | M26035 |
| Species： | Homo sapiens, human |
| Depositors： | BJ Rollins |
| Vector： | Construct size (kb): 3.740000009536743 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.7400000095367432 Tissue: embryonic lung fibroblast WI-38 (serum-induced) Gene product: cytokine A2, small inducible( monocyte chemotactic protein, monocyte chemotactic protein 1, homologous to mouse Sig-je, small inducible cytokine A2, small inducible cytokine subfamily A (Cys-Cys), member 2, small inducible gene JE) JE* |
| Insert Size (kb)： | 0.740 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert includes the following sites (with nucleotide positions according to the published sequence): BglII--193, 286; NcoI--325; PstI--397; SpeI--539. Restriction digests of the clone give the following sizes (kb): XbaI--3.7; BglI--3.7; PstI--3.0, 0.35 (doublet); SacI--3.7; EcoRI/BamHI--3.0, 0.75; XhoI--3.0, 0.75; KpnI--3.0, 0.75. This insert was subcloned from pXM-hJE34, so the KpnI insert includes XhoI, SacI, and PstI sites at the 5 end and XhoI and PstI sites at the 3 end from pXM. The insert includes 24 nucleotides of poly(A) tail. The insert is oriented such that transcription from the SP6 promoter gives the sense strand. |
| References： | Rollins BJ, et al. The human homolog of the JE gene encodes a monocyte secretory protein. Mol. Cell. Biol. 9: 4687-4695, 1989. PubMed: 2513477 Rosl F, et al. Differential regulation of the JE gene encoding the monocyte chemoattractant protein (MCP-1) in cervical carcinoma cells and derived hybrids. J. Virol. 68: 2142-2150, 1994. PubMed: 8138998 Barrett J Rollins, personal communication |
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| 产品名称： | pGEM-hJE34 |
|---|---|
| 商品货号： | TS198632 |
| Designations： | pGEM-hJE34 |
| GenBank Number： | M26035 |
| Species： | Homo sapiens, human |
| Depositors： | BJ Rollins |
| Vector： | Construct size (kb): 3.740000009536743 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.7400000095367432 Tissue: embryonic lung fibroblast WI-38 (serum-induced) Gene product: cytokine A2, small inducible( monocyte chemotactic protein, monocyte chemotactic protein 1, homologous to mouse Sig-je, small inducible cytokine A2, small inducible cytokine subfamily A (Cys-Cys), member 2, small inducible gene JE) JE* |
| Insert Size (kb)： | 0.740 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert includes the following sites (with nucleotide positions according to the published sequence): BglII--193, 286; NcoI--325; PstI--397; SpeI--539. Restriction digests of the clone give the following sizes (kb): XbaI--3.7; BglI--3.7; PstI--3.0, 0.35 (doublet); SacI--3.7; EcoRI/BamHI--3.0, 0.75; XhoI--3.0, 0.75; KpnI--3.0, 0.75. This insert was subcloned from pXM-hJE34, so the KpnI insert includes XhoI, SacI, and PstI sites at the 5 end and XhoI and PstI sites at the 3 end from pXM. The insert includes 24 nucleotides of poly(A) tail. The insert is oriented such that transcription from the SP6 promoter gives the sense strand. |
| References： | Rollins BJ, et al. The human homolog of the JE gene encodes a monocyte secretory protein. Mol. Cell. Biol. 9: 4687-4695, 1989. PubMed: 2513477 Rosl F, et al. Differential regulation of the JE gene encoding the monocyte chemoattractant protein (MCP-1) in cervical carcinoma cells and derived hybrids. J. Virol. 68: 2142-2150, 1994. PubMed: 8138998 Barrett J Rollins, personal communication |
