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pTK-HSV-SCAP-T7(D443N)
pTK-HSV-SCAP-T7(D443N)
规格:
货期:
编号:TS198639
品牌:Testobio
产品名称: pTK-HSV-SCAP-T7(D443N)
商品货号: TS198639
Designations: pTK-HSV-SCAP-T7(D443N)
GenBank Number:

U67060

Species: Cricetus cricetus, hamster
Depositors: JL Goldstein
Applications:
in another host, produces protein SREBP cleavage-activating protein
Vector:

Construct size (kb): 10.0

Intact vector size: 6.0
Type of vector: phagemid
Vector end: ClaI
Vector end: NdeI
Cloning sites: ClaI NdeI
Cloning capacity: pcDNA3, TK, HSV
Host range: mammalian cells; Escherichia coli

Features (with orientation and position when available):
promoter for expression: TK, ->
restriction site: NotI
promoter for in vitro transcription: T7, ->
stop codons: TAG, ->
restriction site: SpeI NheI
initiation codon: ATG, ->
epitope tag: HSV, ->
restriction site: ClaI
restriction site: NdeI
epitope tag: T7, ->
stop codons: TGA, ->
promoter for in vitro transcription: SP6, <-
terminator: bGH polyadenylation, ->
replicon: f1, ->
replicon: SV40, ->
promoter: SV40, ->
marker(s): G418R, ->
terminator: SV40 polyadenylation, ->
replicon: pMB1
marker(s): ampR, <-

Insert:
Genome: Hampster
DNA: cDNA
Insert lengths (kb): 3.8
Gene product: SREBP cleavage-activating protein SCAP*
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Related Products: Distribution host: Escherichia coli HB101 (ATCC 33694)
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments:
Restriction digests of the clone give the following sizes (kb): ClaI--10.0; ClaI/NdeI--6.0, 4.0; NdeI--10.0.
Insert encodes amino acids 2-1276 of the gene with a D443N mutation.
References:

Hua X, et al. Sterol resistance in CHO cells traced to point mutation in SREBP cleavage-activating protein. Cell 87: 415-426, 1996. PubMed: 8898195

Joseph L Goldstein, personal communication

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pTK-HSV-SCAP-T7(D443N)

  • 货号: TS198639
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: pTK-HSV-SCAP-T7(D443N)
商品货号: TS198639
Designations: pTK-HSV-SCAP-T7(D443N)
GenBank Number:

U67060

Species: Cricetus cricetus, hamster
Depositors: JL Goldstein
Applications:
in another host, produces protein SREBP cleavage-activating protein
Vector:

Construct size (kb): 10.0

Intact vector size: 6.0
Type of vector: phagemid
Vector end: ClaI
Vector end: NdeI
Cloning sites: ClaI NdeI
Cloning capacity: pcDNA3, TK, HSV
Host range: mammalian cells; Escherichia coli

Features (with orientation and position when available):
promoter for expression: TK, ->
restriction site: NotI
promoter for in vitro transcription: T7, ->
stop codons: TAG, ->
restriction site: SpeI NheI
initiation codon: ATG, ->
epitope tag: HSV, ->
restriction site: ClaI
restriction site: NdeI
epitope tag: T7, ->
stop codons: TGA, ->
promoter for in vitro transcription: SP6, <-
terminator: bGH polyadenylation, ->
replicon: f1, ->
replicon: SV40, ->
promoter: SV40, ->
marker(s): G418R, ->
terminator: SV40 polyadenylation, ->
replicon: pMB1
marker(s): ampR, <-

Insert:
Genome: Hampster
DNA: cDNA
Insert lengths (kb): 3.8
Gene product: SREBP cleavage-activating protein SCAP*
Media: ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Related Products: Distribution host: Escherichia coli HB101 (ATCC 33694)
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments:
Restriction digests of the clone give the following sizes (kb): ClaI--10.0; ClaI/NdeI--6.0, 4.0; NdeI--10.0.
Insert encodes amino acids 2-1276 of the gene with a D443N mutation.
References:

Hua X, et al. Sterol resistance in CHO cells traced to point mutation in SREBP cleavage-activating protein. Cell 87: 415-426, 1996. PubMed: 8898195

Joseph L Goldstein, personal communication

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