UACC-3199

规格:

货期:

编号:TS198874

品牌:Testobio

产品名称：	UACC-3199
商品货号：	TS198874
Organism：	Homo sapiens, human
Tissue：	mammary gland/breast duct; derived from metastatic site: axillary lymph node
Product Format：	frozen
Morphology：	epithelial-like
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	infiltrating ductal carcinoma of the breast
Age：	58 years old
Gender：	female
Ethnicity：	Caucasian
Applications：	This cell line is an excellent tool for research into aberrant cytosine methylation of CpG island sites and BRCA1 repression in sporadic breast cancer. It is also very useful as a methylated BRCA1 control.
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	UACC-3199 was derived from a 58 year-old female with infiltrating ductal carcinoma of the breast metastatic to the left axillary lymph nodes.xa0
Clinical Data：	female The patient had a family history of breast cancer. 58 years old Caucasian
Receptor Expression：	epidermal growth factor receptor (EGF), expressed estrogen receptor, not expressed progesterone receptor, not expressed
Oncogene：	c-erbB-2 positive
Genes Expressed：	c-erbB-2 positive
Tumorigenic：	YES
Comments：	The patient had a family history of breast cancer.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium (ATCC^® 30-2008). To make the complete growth medium, add the following components to the base medium:xa0 fetal bovine serum to a final concentration of 5% 0.01 mg/mL transferrin (final conc.) 0.01 mg/mL insulin (final conc.) 5 µg/mL (55 U/mL) catalase (final conc.) 3.6 µg/mL (0.01 mM) hydrocortisone (final conc.) extra 2 mM glutamine
Subculturing：	Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 1 X 10⁴ to 2 X 10⁴ viable cells/cm² is recommended. Incubate cultures at 37°C. Subculture when the cell concentration is between 2 X 10⁴ to 5 X 10⁴ cells/cm². Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium renewal: Every 3 to 4 days Growth Conditions:xa0Cells grow very slowly. Cultures reach confluence in 2 to 3 weeks. Avoid inoculating vessels below recommended seeding density.
Cryopreservation：	Freeze medium: complete growth medium, 90%; DMSO, 10% Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°C Atmosphere: air, 100%
STR Profile：	CSF1PO: 12 D13S317: 11 D16S539: 12 D5S818: 13 D7S820: 10 THO1: 9.3 TPOX: 10, 13 vWA: 15, 17 Amelogenin: X
Name of Depositor：	K Brown
Year of Origin：	November 1994
References：	Rice JC, et al. Transcriptional repression of BRCA1 by aberrant cytosine methylation, histone hypoacetylation and chromatin condensation of the BRCA1 promoter. Nucleic Acids Res. 28(17): 3233-3239, 2000. PubMed: 10954590 Rice JC, et al. Aberrant methylation of the BRCA1 CpG island promoter is associated with decreased BRCA1 mRNA in sporadic breast cancer cells. Oncogene 17(14): 1807-1812, 1998. PubMed: 9778046

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UACC-3199

货号: TS198874
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产品名称：	UACC-3199
商品货号：	TS198874
Organism：	Homo sapiens, human
Tissue：	mammary gland/breast duct; derived from metastatic site: axillary lymph node
Product Format：	frozen
Morphology：	epithelial-like
Culture Properties：	adherent
Biosafety Level：	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease：	infiltrating ductal carcinoma of the breast
Age：	58 years old
Gender：	female
Ethnicity：	Caucasian
Applications：	This cell line is an excellent tool for research into aberrant cytosine methylation of CpG island sites and BRCA1 repression in sporadic breast cancer. It is also very useful as a methylated BRCA1 control.
Storage Conditions：	liquid nitrogen vapor phase
Images：
Derivation：	UACC-3199 was derived from a 58 year-old female with infiltrating ductal carcinoma of the breast metastatic to the left axillary lymph nodes.xa0
Clinical Data：	female The patient had a family history of breast cancer. 58 years old Caucasian
Receptor Expression：	epidermal growth factor receptor (EGF), expressed estrogen receptor, not expressed progesterone receptor, not expressed
Oncogene：	c-erbB-2 positive
Genes Expressed：	c-erbB-2 positive
Tumorigenic：	YES
Comments：	The patient had a family history of breast cancer.
Complete Growth Medium：	The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium (ATCC^® 30-2008). To make the complete growth medium, add the following components to the base medium:xa0 fetal bovine serum to a final concentration of 5% 0.01 mg/mL transferrin (final conc.) 0.01 mg/mL insulin (final conc.) 5 µg/mL (55 U/mL) catalase (final conc.) 3.6 µg/mL (0.01 mM) hydrocortisone (final conc.) extra 2 mM glutamine
Subculturing：	Volumes used in this protocol are for 75 cm² flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 1 X 10⁴ to 2 X 10⁴ viable cells/cm² is recommended. Incubate cultures at 37°C. Subculture when the cell concentration is between 2 X 10⁴ to 5 X 10⁴ cells/cm². Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium renewal: Every 3 to 4 days Growth Conditions:xa0Cells grow very slowly. Cultures reach confluence in 2 to 3 weeks. Avoid inoculating vessels below recommended seeding density.
Cryopreservation：	Freeze medium: complete growth medium, 90%; DMSO, 10% Storage temperature: liquid nitrogen vapor phase
Culture Conditions：	Temperature: 37°C Atmosphere: air, 100%
STR Profile：	CSF1PO: 12 D13S317: 11 D16S539: 12 D5S818: 13 D7S820: 10 THO1: 9.3 TPOX: 10, 13 vWA: 15, 17 Amelogenin: X
Name of Depositor：	K Brown
Year of Origin：	November 1994
References：	Rice JC, et al. Transcriptional repression of BRCA1 by aberrant cytosine methylation, histone hypoacetylation and chromatin condensation of the BRCA1 promoter. Nucleic Acids Res. 28(17): 3233-3239, 2000. PubMed: 10954590 Rice JC, et al. Aberrant methylation of the BRCA1 CpG island promoter is associated with decreased BRCA1 mRNA in sporadic breast cancer cells. Oncogene 17(14): 1807-1812, 1998. PubMed: 9778046