pHGH-1 [HGH X-1776]
pHGH-1 [HGH X-1776]
规格:
货期:
编号:TS199249
品牌:Testobio
| 产品名称： | pHGH-1 HGH X-1776 |
|---|---|
| 商品货号： | TS199249 |
| Designations： | pHGH-1 HGH X-1776 |
| GenBank Number： | I03167 |
| Species： | Homo sapiens, human |
| Depositors： | University of California, Davis |
| Applications： | For growth of the host strain, the medium must contain DAP (diaminopimelic acid) (100 mg/L) and thymidine (20 mg/L). |
| Vector： | Construct size (kb): 5.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: HindIII Vector end: HindIII Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: GH1 Genomic copy number: unique Gene name: growth hormone 1 Contains complete coding sequence?: U Chromosome: 17; Localization: 17 q22-q24 Tissue: pituitary neoplasm Type of DNA: cDNA Insert end: Modification: HindIII linkers Insert end: Modification: HindIII linkers Insert size (kb): 0.55 Cross references: DNA Seq. Acc.: I03167 Insert lengths(kb): 0.550000011920929 Tissue: pituitary neoplasm Gene product: growth hormone 1(growth hormone GH1) GH1 |
| Insert Size (kb)： | 0.550 |
| Media： | ATCC® Medium 1083: LB medium for chi 1776 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.5; PstI--4.5, 0.72. For growth of the host strain, the medium must contain DAP (diaminopimelic acid) (100 mg/L) and thymidine (20 mg/L). Contains sequences encoding aa 24-191 of the protein, plus 3 untranslated sequence. |
| References： | Goodman HM, et al. Recombinant DNA transfer vectors. US Patent 4,363,877 dated Dec 14 1982 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
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pHGH-1 [HGH X-1776]
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| 产品名称： | pHGH-1 HGH X-1776 |
|---|---|
| 商品货号： | TS199249 |
| Designations： | pHGH-1 HGH X-1776 |
| GenBank Number： | I03167 |
| Species： | Homo sapiens, human |
| Depositors： | University of California, Davis |
| Applications： | For growth of the host strain, the medium must contain DAP (diaminopimelic acid) (100 mg/L) and thymidine (20 mg/L). |
| Vector： | Construct size (kb): 5.0 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBR322 Intact vector size: 4.363 Type of vector: plasmid Vector end: HindIII Vector end: HindIII Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI PstI PvuI ScaI SspI AatII Polylinker sites: Construction: pBR313 Host range: Escherichia coli Features (with orientation and position when available): marker(s): tetR replicon: pMB1 marker(s): ampR Cross references: DNA Seq. Acc.: J01749 |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: human Gene symbol: GH1 Genomic copy number: unique Gene name: growth hormone 1 Contains complete coding sequence?: U Chromosome: 17; Localization: 17 q22-q24 Tissue: pituitary neoplasm Type of DNA: cDNA Insert end: Modification: HindIII linkers Insert end: Modification: HindIII linkers Insert size (kb): 0.55 Cross references: DNA Seq. Acc.: I03167 Insert lengths(kb): 0.550000011920929 Tissue: pituitary neoplasm Gene product: growth hormone 1(growth hormone GH1) GH1 |
| Insert Size (kb)： | 0.550 |
| Media： | ATCC® Medium 1083: LB medium for chi 1776 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--5.5; PstI--4.5, 0.72. For growth of the host strain, the medium must contain DAP (diaminopimelic acid) (100 mg/L) and thymidine (20 mg/L). Contains sequences encoding aa 24-191 of the protein, plus 3 untranslated sequence. |
| References： | Goodman HM, et al. Recombinant DNA transfer vectors. US Patent 4,363,877 dated Dec 14 1982 |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
