你好,请登录   免费注册    |    收藏本站
联系电话: 0574-87917803
联系电话: call_new 0574-87917803
MEF (CF-1) IRR
MEF (CF-1) IRR
规格:
货期:
编号:TS199343
品牌:Testobio
产品名称: MEF (CF-1) IRR
商品货号: TS199343
Organism: Mus musculus, mouse
Tissue: Embryo
Cell Type: Fibroblast
Product Format: frozen
Morphology: Fibroblast
Culture Properties: Adherent
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age: 14 days gestation embryo
Gender: male and female mixed
Strain: CF-1, non-inbred mouse strain (non-agouti albino) from Carworth Farms
Applications:
Irradiated cells for use as feeder layer
Storage Conditions: liquid nitrogen vapor phase
Clinical Data:
male and female mixed
Comments: These cells have been growth-arrested by irradiation with 10,000 rads. The cells will begin to deteriorate 7 to 10 days after plating and may no longer support the growth of other cells. We recommend that you do not keep the cells in culture for longer than 7 to 10 days.
Complete Growth Medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 15%

  • This medium is formulated for use with a 5% CO2 in air atmosphere. (Standard DMEM formulations contain 3.7 g/L sodium bicarbonate and a 10% CO2 in air atmosphere is then recommended).
    Subculturing:
    Medium Renewal: Twice a week or when pH decreases
    Culture Conditions:
    Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37°C
    Name of Depositor: ATCC
    Year of Origin: 2004
    References:

    Nagy A, et al. Manipulating The Mouse Embryo: A Laboratory Manual. Third Edition: Cold Spring Harbor Press; 2003.

    首页 > 产品中心 > 微生物培养 > 菌株 > null > MEF (CF-1) IRR

    MEF (CF-1) IRR

    • 货号: TS199343
    • 好评
    询价
    • 品牌 : TESTOBIO
    产品名称: MEF (CF-1) IRR
    商品货号: TS199343
    Organism: Mus musculus, mouse
    Tissue: Embryo
    Cell Type: Fibroblast
    Product Format: frozen
    Morphology: Fibroblast
    Culture Properties: Adherent
    Biosafety Level: 1

    Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

    Age: 14 days gestation embryo
    Gender: male and female mixed
    Strain: CF-1, non-inbred mouse strain (non-agouti albino) from Carworth Farms
    Applications:
    Irradiated cells for use as feeder layer
    Storage Conditions: liquid nitrogen vapor phase
    Clinical Data:
    male and female mixed
    Comments: These cells have been growth-arrested by irradiation with 10,000 rads. The cells will begin to deteriorate 7 to 10 days after plating and may no longer support the growth of other cells. We recommend that you do not keep the cells in culture for longer than 7 to 10 days.
    Complete Growth Medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 15%

  • This medium is formulated for use with a 5% CO2 in air atmosphere. (Standard DMEM formulations contain 3.7 g/L sodium bicarbonate and a 10% CO2 in air atmosphere is then recommended).
    Subculturing:
    Medium Renewal: Twice a week or when pH decreases
    Culture Conditions:
    Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37°C
    Name of Depositor: ATCC
    Year of Origin: 2004
    References:

    Nagy A, et al. Manipulating The Mouse Embryo: A Laboratory Manual. Third Edition: Cold Spring Harbor Press; 2003.

    合作单位: