| 产品名称： | Adeno-associated virus 1 |
|---|---|
| 商品货号： | TS199930 |
| Classification： | Parvoviridae, Dependovirus, Adeno-associated virus-1 |
| Deposited As： | Adeno-associated (satellite) virus type 1 |
| Agent： | Adeno-associated virus 1 |
| Strain： | none |
| Applications： | Suggest VR-848, Adenovirus Type 7A, as helper virus, which produces CPE on ATCC CRL-1573 cells. |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Pool of RhMk TC infected with SV-15 virus |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder (or colder) |
| Comments： | Depends upon adenovirus for replication. Suggest VR-848, Adenovirus Type 7A, as helper virus, which produces CPE on ATCC CRL-1573 cells. *Helper virus may be inactivated by heating to 56C for 30 min. |
| Effect on Host： | in vitro effects: Adeno-associated virus shows no independent cytopathic effects Adenovirus CPE, refractile rounding and sloughing; AAV shows no independent CPE. Yes, in vitro effects: Adenovirus cytopathic effects, refractile rounding and sloughing |
| Growth Medium： | Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
|
| Recommended Host： | Recommended Host:xa0 HEK-293 (ATCC® CRL-1573TM) |
| Growth Conditions： | Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C Duration: 2-4 days; For best results cells should be 24 to 48 hours old and 70-90% confluent (not 100% confluent). |
| Cryopreservation： | Storage temperature: -70°C |
| Name of Depositor： | WP Parks |
| Special Collection： | ATCC |
| Source： | Pool of RhMk TC infected with SV-15 virus |
| References： | Atchison RW, et al. Adenovirus-associated defective virus particles. Science 149: 754-756, 1965. PubMed: 14325163 or colder or colder |
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| 产品名称： | Adeno-associated virus 1 |
|---|---|
| 商品货号： | TS199930 |
| Classification： | Parvoviridae, Dependovirus, Adeno-associated virus-1 |
| Deposited As： | Adeno-associated (satellite) virus type 1 |
| Agent： | Adeno-associated virus 1 |
| Strain： | none |
| Applications： | Suggest VR-848, Adenovirus Type 7A, as helper virus, which produces CPE on ATCC CRL-1573 cells. |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | Pool of RhMk TC infected with SV-15 virus |
| Product Format： | frozen |
| Storage Conditions： | -70°C or colder (or colder) |
| Comments： | Depends upon adenovirus for replication. Suggest VR-848, Adenovirus Type 7A, as helper virus, which produces CPE on ATCC CRL-1573 cells. *Helper virus may be inactivated by heating to 56C for 30 min. |
| Effect on Host： | in vitro effects: Adeno-associated virus shows no independent cytopathic effects Adenovirus CPE, refractile rounding and sloughing; AAV shows no independent CPE. Yes, in vitro effects: Adenovirus cytopathic effects, refractile rounding and sloughing |
| Growth Medium： | Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
|
| Recommended Host： | Recommended Host:xa0 HEK-293 (ATCC® CRL-1573TM) |
| Growth Conditions： | Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C Duration: 2-4 days; For best results cells should be 24 to 48 hours old and 70-90% confluent (not 100% confluent). |
| Cryopreservation： | Storage temperature: -70°C |
| Name of Depositor： | WP Parks |
| Special Collection： | ATCC |
| Source： | Pool of RhMk TC infected with SV-15 virus |
| References： | Atchison RW, et al. Adenovirus-associated defective virus particles. Science 149: 754-756, 1965. PubMed: 14325163 or colder or colder |
