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HHCPN17
HHCPN17
规格:
货期:
编号:TS200067
品牌:Testobio
产品名称: HHCPN17
商品货号: TS200067
Designations: HHCPN17
GenBank Number:

X15309

M78157

Species: Homo sapiens, human
Vector:
Construct size (kb): 4.099999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: NEFH
Genomic copy number: unique
Gene name: neurofilament, heavy polypeptide (200kD)
Contains complete coding sequence?: N
Chromosome: 22; Localization: 22 q12.1-q13.1
Tissue: hippocampus, female, 2 years, oligo-dT and random primed
(Stratagene 936205), subtracted
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.2
Cross references: DNA Seq. Acc.: M78157; EST: EST01749; Primer: M13 Forward;
THC id: THC76212
DNA Seq. Acc.: X15309; EST: EST01749
Nucleotides 1-357 of the insert correspond to
nucleotides 2425-2069 of X15309 with 99% identity.
Insert lengths(kb): 1.200000047683716
Tissue: hippocampus, female, 2 years, oligo-dT and random primed (Stratagene 936205), subtracted
Gene product: neurofilament, heavy polypeptide (200kD)(neurofilament, heavy polypeptide) NEFH
Insert Size (kb): 1.200
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.2; BamHI--4.1; PstI--4.1.
Based on the size of the insert and the alignment with GenBank X15309 (2542 nt, exon 4), this clone appears to contain part of exon 4.
References:

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

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HHCPN17

  • 货号: TS200067
  • 好评
询价
  • 品牌 : TESTOBIO
产品名称: HHCPN17
商品货号: TS200067
Designations: HHCPN17
GenBank Number:

X15309

M78157

Species: Homo sapiens, human
Vector:
Construct size (kb): 4.099999904632568
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:
Insert:
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: NEFH
Genomic copy number: unique
Gene name: neurofilament, heavy polypeptide (200kD)
Contains complete coding sequence?: N
Chromosome: 22; Localization: 22 q12.1-q13.1
Tissue: hippocampus, female, 2 years, oligo-dT and random primed
(Stratagene 936205), subtracted
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.2
Cross references: DNA Seq. Acc.: M78157; EST: EST01749; Primer: M13 Forward;
THC id: THC76212
DNA Seq. Acc.: X15309; EST: EST01749
Nucleotides 1-357 of the insert correspond to
nucleotides 2425-2069 of X15309 with 99% identity.
Insert lengths(kb): 1.200000047683716
Tissue: hippocampus, female, 2 years, oligo-dT and random primed (Stratagene 936205), subtracted
Gene product: neurofilament, heavy polypeptide (200kD)(neurofilament, heavy polypeptide) NEFH
Insert Size (kb): 1.200
Biosafety Level: 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information: Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments:
Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.2; BamHI--4.1; PstI--4.1.
Based on the size of the insert and the alignment with GenBank X15309 (2542 nt, exon 4), this clone appears to contain part of exon 4.
References:

Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749

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